Inhibition of soybean urease by triketone oximes
| Autor: | D. B. Rubinov, E. I. Tarun, D. I. Metelitza |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Urease
Substituent chemistry.chemical_element Biochemistry Medicinal chemistry Substrate Specificity chemistry.chemical_compound Residue (chemistry) Non-competitive inhibition Oximes Organic chemistry Enzyme Inhibitors Alkyl chemistry.chemical_classification Aqueous solution Molecular Structure biology General Medicine Hydrogen-Ion Concentration Ketones Oxime Kinetics Nickel chemistry biology.protein Soybeans |
| Zdroj: | Biochemistry (Moscow). 69:1344-1352 |
| ISSN: | 1608-3040 0006-2979 |
| Popis: | Competitive inhibition of soybean urease by 15 triketone oximes has been studied at 36°C in aqueous solution (pH 4.95). The studied oximes are supposed chelators for the nickel atom in the urease metallocenter. The inhibition constants of urea hydrolysis (K i) varied in the range 2.7-248 μM depending on the oxime structure. Analysis of this dependency demonstrates that the optimal inhibitor is the one containing carbonyl group in position 1 of the cycle, the ethoxyimino group and alkyl residue in the substituent in position 2, as well as the methoxycarbonyl group in position 4 of the cycle. |
| Databáze: | OpenAIRE |
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