In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa
| Autor: | Rui Sun, Juan Kan, Yao Wang, Nianfeng Zhang, Chunlu Qian, Xin Zhang, Jun Liu, Changhai Jin, Sixue Tang, Xiaonan Wu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Male Cell Survival medicine.drug_class medicine.medical_treatment Anti-Inflammatory Agents 02 engineering and technology Gut flora digestive system Biochemistry Anti-inflammatory Microbiology Proinflammatory cytokine Mice 03 medical and health sciences Structural Biology In vivo medicine Animals Molecular Biology 030304 developmental biology Mice Inbred ICR 0303 health sciences biology Chemistry musculoskeletal neural and ocular physiology Body Weight Water General Medicine Fatty Acids Volatile 021001 nanoscience & nanotechnology biology.organism_classification Arctium Gastrointestinal Microbiome RAW 264.7 Cells Cytokine Gene Expression Regulation Solubility Arctium lappa Cytokines Tumor necrosis factor alpha Bacteroides 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 135:717-724 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.05.171 |
| Popis: | In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice. Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α). Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group. There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium. Simultaneously, symbiotic bacteria like Bacteroides were inhibited by ALP-1. Besides, ALP-1 could significantly enhance the production of short chain fatty acids (SCFAs) in gut. |
| Databáze: | OpenAIRE |
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