Single-cell reconstruction of follicular remodeling in the human adult ovary
| Autor: | Hailiang Mei, Vanessa Torrens-Juaneda, Monika Bialecka, Eric Lam, Ioannis Moustakas, N. V. Borggreven, L. A. Louwe, L. van der Westerlaken, S M Chuva de Sousa Lopes, Gonneke S. K. Pilgram, Xueying Fan, L. Trouw |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell General Physics and Astronomy 02 engineering and technology PROGRAMS Ovarian Follicle Follicular phase Medicine and Health Sciences lcsh:Science GENE-EXPRESSION Multidisciplinary CUMULUS Theca Cell 021001 nanoscience & nanotechnology FLUID Cell biology GRANULOSA-CELLS medicine.anatomical_structure DIFFERENTIATION Mechanisms of disease Theca Theca Cells OOCYTE MATURATION Female Folliculogenesis 0210 nano-technology C1Q RECRUITMENT Adult Ovulation endocrine system Stromal cell Science Myocytes Smooth Muscle Ovary Biology General Biochemistry Genetics and Molecular Biology Article Functional clustering 03 medical and health sciences EMBRYOS medicine Humans Innate immune system Granulosa Cells Base Sequence Sequence Analysis RNA Uterus Endothelial Cells General Chemistry 030104 developmental biology lcsh:Q |
| Zdroj: | Nature Communications NATURE COMMUNICATIONS Nature Communications, 10 Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2041-1723 |
| Popis: | The ovary is perhaps the most dynamic organ in the human body, only rivaled by the uterus. The molecular mechanisms that regulate follicular growth and regression, ensuring ovarian tissue homeostasis, remain elusive. We have performed single-cell RNA-sequencing using human adult ovaries to provide a map of the molecular signature of growing and regressing follicular populations. We have identified different types of granulosa and theca cells and detected local production of components of the complement system by (atretic) theca cells and stromal cells. We also have detected a mixture of adaptive and innate immune cells, as well as several types of endothelial and smooth muscle cells to aid the remodeling process. Our results highlight the relevance of mapping whole adult organs at the single-cell level and reflect ongoing efforts to map the human body. The association between complement system and follicular remodeling may provide key insights in reproductive biology and (in)fertility. In the ovary, follicular degeneration occurs after folliculogenesis, with one dominant follicle reaching maturity every month. Here, by performing single cell RNA-seq of adult human follicles, the authors identify subclasses of granulosa and theca cell lineages, which correlate with the growth or degeneration of the follicles. |
| Databáze: | OpenAIRE |
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