A preliminary study procedure for detection of polyamines in plasma samples as a potential diagnostic tool in prostate cancer
| Autor: | Tatiana Solinas, Emanuela Azara, Ciriaco Carru, Massimo Madonia, Caterina Arru, Angelo Zinellu, Serenella Medici, Donatella Coradduzza |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Analyte Clinical Biochemistry Orbitrap Mass spectrometry Biochemistry Analytical Chemistry law.invention Prostate cancer law Limit of Detection Tandem Mass Spectrometry medicine Biomarkers Tumor Polyamines Humans UHPLCOrbitrap mass spectrometer Chromatography High Pressure Liquid Detection limit Chromatography Plasma samples Chemistry Prostatic Neoplasms Reproducibility of Results Cell Biology General Medicine medicine.disease Linear Models Plasma polyamines Quantitative analysis (chemistry) |
| Zdroj: | Journal of chromatography. B 1162 (2021). doi:10.1016/j.jchromb.2020.122468 info:cnr-pdr/source/autori:Donatella Coradduzza, Emanuela Azara, Serenella Medici, Caterina Arru, Tatiana Solinas, Massimo Madonia, Angelo Zinellu and Ciriaco Carru/titolo:A preliminary study procedure for detection of polyamines in plasma samples as a potential diagnostic tool in prostate cancer/doi:10.1016%2Fj.jchromb.2020.122468/rivista:Journal of chromatography. B (Print)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:1162 |
| DOI: | 10.1016/j.jchromb.2020.122468 |
| Popis: | Background Many scientific contributions recognize polyamines as important biomarkers for the diagnosis and treatment of cancer. Several authors have suggested the use of LC/MS instruments as an elective method for their measurement, providing good detection limits and specificity; however, many of these procedures suffer from long chromatographic run times, high detection limits and lengthy and expensive sample pre-treatment steps. Methods UHPLC coupled with high-resolution Orbitrap mass spectrometry (UHPLC/Orbitrap) was set up for the identification and separation ofpolyamines, together with some of their metabolites and catabolites, in the plasma of healthy and prostate cancer human patients. Thirteen metabolites were measured in deproteinized plasma samples through a new analytical approach known as the parallel reaction monitoring (PRM) for targeted quantitative analysis. Results The calibration curves were linear and R2 ranged from 0.9913 to 0.9995 for all analytes. LOQ values are between 0.382 and 25 ng mL-1 and LOD values are between 0.109 and 7.421 ng mL-1. The method shows an accuracy and precision for intra-day and inter-day < 15% RSD and R.E.% for all the QC samples. The matrix effect calculated at different concentration levels did not exceed 15%. Conclusions The method developed provides rapid, easy and robust identification and measurement of a wide range of polyamines, and some of their metabolites that can be evaluated as biomarkers to predict the clinical features of prostate cancer patients, avoiding invasive diagnostic procedures. |
| Databáze: | OpenAIRE |
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