The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM
| Autor: | Karl M. Thompson, Shiyun Chen, Marcus Fredriksson Sundbom, Ikenna Obi, Monika K. Francis, Matthew S. Francis, Dharmender Kumar Gahlot, Petra Dersch, Kristina Ruuth, Junfa Liu, Jyoti M. Gurung, Edvin J. Thanikkal |
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| Přispěvatelé: | HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. |
| Rok vydání: | 2019 |
| Předmět: |
Transcriptional Activation
Microbiology (medical) microbial behaviour Immunology Biology Yersinia Microbiology lcsh:Infectious and parasitic diseases Microbiology in the medical area Environmental stress responsiveness 03 medical and health sciences Bacterial Proteins gene expression control Stress Physiological Transcriptional regulation Mikrobiologi inom det medicinska området Yersinia pseudotuberculosis lcsh:RC109-216 Phosphorylation growth and survival 030304 developmental biology 0303 health sciences Virulence 030306 microbiology Kinase Mutagenesis Promoter Gene Expression Regulation Bacterial biology.organism_classification Cell biology fitness Response regulator Mikrobiologi Infectious Diseases metabolic networks Parasitology Transcription Factors Research Paper |
| Zdroj: | Virulence Virulence, Vol 10, Iss 1, Pp 37-57 (2019) |
| DOI: | 10.6084/m9.figshare.7428893 |
| Popis: | The Gram-negative enteropathogen Yersinia pseudotuberculosis possesses a number of regulatory systems that detect cell envelope damage caused by noxious extracytoplasmic stresses. The CpxA sensor kinase and CpxR response regulator two-component regulatory system is one such pathway. Active Cpx signalling upregulates various factors designed to repair and restore cell envelope integrity. Concomitantly, this pathway also down-regulates key determinants of virulence. In Yersinia, cpxA deletion accumulates high levels of phosphorylated CpxR (CpxR~P). Accumulated CpxR~P directly repressed rovA expression and this limited expression of virulence-associated processes. A second transcriptional regulator, RovM, also negatively regulates rovA expression in response to nutrient stress. Hence, this study aimed to determine if CpxR~P can influence rovA expression through control of RovM levels. We determined that the active CpxR~P isoform bound to the promoter of rovM and directly induced its expression, which naturally associated with a concurrent reduction in rovA expression. Site-directed mutagenesis of the CpxR~P binding sequence in the rovM promoter region desensitised rovM expression to CpxR~P. These data suggest that accumulated CpxR~P inversely manipulates the levels of two global transcriptional regulators, RovA and RovM, and this would be expected to have considerable influence on Yersinia pathophysiology and metabolism. |
| Databáze: | OpenAIRE |
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