Use of monoclonal and polyclonal antibodies as structural and topographical probes for hepatic epoxide hydrolase
| Autor: | Christopher Timms, Franz Oesch, Monika Maruhn, Renate Hartmann, Thomas M. Guenthner, Reinhard Burger, C.Roland Wolf |
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| Rok vydání: | 1983 |
| Předmět: |
Male
Primates Monoclonal antibody Immunodiffusion medicine.drug_class Guinea Pigs Biophysics Hamster Epoxide hydrolase Biochemistry Antibodies Mice Enzyme-linked immunosorbent assay Structural Biology Cricetinae Genetics medicine Animals Humans Molecular Biology Epoxide Hydrolases biology Chemistry Endoplasmic reticulum Antibodies Monoclonal Rats Inbred Strains Cell Biology Molecular biology Rats Liver Polyclonal antibodies Membrane topology Monoclonal Protein structure biology.protein Epoxy Compounds Rabbits Antibody |
| Zdroj: | FEBS Letters. 157:271-276 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(83)80560-2 |
| Popis: | Monoclonal antibodies have been prepared against rat liver epoxide hydrolase (EH), some of which gave precipitation lines on immunodiffusion against pure EH suggesting the presence of repetitive structural domains on the enzyme. Using ELISA, with polyclonal antibodies to rat and rabbit liver EH, reactivity and therefore structural similarities between EH of all species tested, including human, were observed. This was in contrast to immunodiffusion results demonstrating the limitations of the latter technique. Using monoclonal antibodies in ELISA, greatest structural similarity was between rat, mouse, and Syrian hamster EH and relatively little between rat and human. Two of the antibodies reacted with nearly all species tested and may be directed towards critical sites on the enzyme. This and most of the EH molecule would appear to be localised on the cytoplasmic surface of the endoplasmic reticulum. |
| Databáze: | OpenAIRE |
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