Preliminary proteomic-based identification of a novel protein for Down's syndrome in maternal serum
| Autor: | Bin Zhang, Shi-he Shao, Qiu-wei Wang, Jing Wang, Rui-ping Huang, Bin Yu, Yuqi Yang |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Proteomics Immunoglobulins Prenatal diagnosis Biology Complement factor B General Biochemistry Genetics and Molecular Biology Desmin Andrology Young Adult Enzyme regulator activity Pregnancy Prenatal Diagnosis Humans Serum amyloid P component Glycoproteins Transferrin Ceruloplasmin Molecular biology Serum Amyloid P-Component Case-Control Studies Pregnancy Trimester Second alpha 1-Antitrypsin Proteome biology.protein Female Down Syndrome Biological regulation Biomarkers |
| Zdroj: | Experimental biology and medicine (Maywood, N.J.). 237(5) |
| ISSN: | 1535-3699 |
| Popis: | Prenatal screening for Down's syndrome (DS) is in need of improvement. As a powerful platform, proteomics techniques could also be used for identification of new biomarkers for DS screening. In this case-control proteome study, pregnant women were diagnosed prenatally by karyotype analysis from amniotic fluid (AF). Maternal serum samples were collected from six pregnancies with fetuses affected by DS and six pregnancies with normal fetuses. First, we used two-dimensional electrophoresis and mass spectrometry to identify the different levels of expression of proteins in maternal serum between the DS and control groups in the second trimester. Second, we used bioinformatics to analyze the proteins by DAVID. Then, the interesting candidates were further tested by enzyme-linked immunosorbent assay (ELISA). Twenty-nine proteins were successfully identified in maternal serum obtained from pregnancies with fetuses affected by DS. The top five proteins up-regulated were serotransferrin (TF), alpha-1b-glycoprotein (A1BG), desmin (DES), alpha-1-antitrypsin (SERPINA1) and ceruloplasmin (CP), while serum amyloid P-component (APCS) was the most down-regulated protein. These 29 proteins were categorized based on binding, catalytic activity and enzyme regulator activity. The biological roles were involved in biological regulation, metabolic processes, cellular processes and response to a stimulus. Based on ELISA, the median concentrations of CP and complement factor B (CFB) were 332.3 and 412.3 ng/mL, respectively. The concentrations of CP and CFB were significantly higher in the DS group than in the control group ( P < 0.05). In conclusion, proteomic approaches offer the possibility of further improving the performance of DS screening and our identification of up- and down-regulated proteins may lead to new candidates for DS screening. |
| Databáze: | OpenAIRE |
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