Bone regeneration mediated by biomimetic mineralization of a nanofiber matrix
| Autor: | Robert L. Satcher, Karl Henrikson, Conrado Aparicio, Samuel I. Stupp, Stuart R. Stock, Alvaro Mata, Yanbiao Geng |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Bone Regeneration Materials science Nanofibers Biophysics Bioengineering Peptide Mineralization (biology) Regenerative medicine Article Rats Sprague-Dawley Biomaterials chemistry.chemical_compound Calcification Physiologic Peptide amphiphile Animals Bone regeneration Tomography chemistry.chemical_classification Molecular Mimicry Rats chemistry Mechanics of Materials Nanofiber Phosphoserine Microscopy Electron Scanning Ceramics and Composites Biomedical engineering Biomineralization |
| Zdroj: | Biomaterials. 31:6004-6012 |
| ISSN: | 0142-9612 |
| Popis: | Rapid bone regeneration within a three-dimensional defect without the use of bone grafts, exogenous growth factors, or cells remains a major challenge. We report here on the use of self-assembling peptide nanostructured gels to promote bone regeneration that have the capacity to mineralize in biomimetic fashion. The main molecular design was the use of phosphoserine residues in the sequence of a peptide amphiphile known to nucleate hydroxyapatite crystals on the surfaces of nanofibers. We tested the system in a rat femoral critical-size defect by placing pre-assembled nanofiber gels in a 5 mm gap and analyzed bone formation with micro-computed tomography and histology. We found within 4 weeks significantly higher bone formation relative to controls lacking phosphorylated residues and comparable bone formation to that observed in animals treated with a clinically used allogenic bone matrix. |
| Databáze: | OpenAIRE |
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