Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin

Autor:	Gregorino Paone, Massimo Amicosante, G. Trenti, Cesare Saltini, A. Bisetti, Mario Campa, Luca Richeldi
Předmět:	Microbiology (medical) Tuberculosis Molecular Sequence Data DNA-Directed DNA Polymerase Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Mycobacterium tuberculosis chemistry.chemical_compound law medicine Humans Anion binding Anion Exchange Resins Polymerase Polymerase chain reaction Settore MED/04 - Patologia Generale Mycobacterium bovis Base Sequence biology Sputum Settore MED/07 - Microbiologia e Microbiologia Clinica biology.organism_classification medicine.disease PCR tuberculosis chemistry biology.protein medicine.symptom DNA Research Article
Zdroj:	Scopus-Elsevier
Popis:	Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).
Databáze:	OpenAIRE
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