Healing of Preterm Ruptured Fetal Membranes
Autor: | Haruta Mogami, R. Ann Word, Annavarapu Hari Kishore, Yucel Akgul |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Fetal Membranes Premature Rupture Epithelial-Mesenchymal Transition Amniotic fluid Interleukin-1beta lcsh:Medicine Epithelial cell migration Article Proinflammatory cytokine Andrology Mice 03 medical and health sciences Cell Movement Pregnancy Fetal membrane Animals Medicine lcsh:Science Wound Healing Fetus Multidisciplinary Amnion Tumor Necrosis Factor-alpha business.industry Macrophages Mesenchymal stem cell lcsh:R Epithelial Cells medicine.disease 030104 developmental biology medicine.anatomical_structure embryonic structures Female lcsh:Q business Premature rupture of membranes |
Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Preterm premature rupture of membrane (pPROM) is associated with 30–40% of preterm births. Infection is considered a leading cause of pPROM due to increased levels of proinflammatory cytokines in amniotic fluid. Only 30%, however, are positive for microbial organisms by amniotic fluid culture. Interestingly, in some pregnancies complicated by preterm premature rupture of membranes (pPROM), membranes heal spontaneously and pregnancy continues until term. Here, we investigated mechanisms of amnion healing. Using a preclinical mouse model, we found that small ruptures of the fetal membrane closed within 72 h whereas healing of large ruptures was only 40%. Small rupture induced transient upregulation of cytokines whereas large ruptures elicited sustained upregulation of proinflammatory cytokines in the fetal membranes. Fetal macrophages from amniotic fluid were recruited to the wounded amnion where macrophage adhesion molecules were highly expressed. Recruited macrophages released limited and well-localized amounts of IL-1β and TNF which facilitated epithelial-mesenchymal transition (EMT) and epithelial cell migration. Arg1 + macrophages dominated within 24 h. Migration and healing of the amnion mesenchymal compartment, however, remained compromised. These findings provide novel insights regarding unique healing mechanisms of amnion. |
Databáze: | OpenAIRE |
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