Enhanced and coordinated in vivo expression of inflammatory cytokines and nitric oxide synthase by chondrocytes from patients with osteoarthritis
| Autor: | Tania Silvestri, Mariagrazia Uguccioni, Rosa Maria Borzì, Riccardo Meliconi, Ilaria Mazzetti, Cinzia Melchiorri, Andrea Facchini, Lia Pulsatelli, L. Frizziero |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Cartilage Articular Male Pathology medicine.medical_specialty medicine.medical_treatment Immunology Arthritis Nitric Oxide Synthase Type II Inflammation Osteoarthritis Chondrocyte Proinflammatory cytokine Rheumatology medicine Immunology and Allergy Humans Pharmacology (medical) Aged business.industry Tumor Necrosis Factor-alpha Synovial Membrane Middle Aged medicine.disease medicine.anatomical_structure Cytokine Cytokines Tumor necrosis factor alpha Female Synovial membrane medicine.symptom Inflammation Mediators Nitric Oxide Synthase business Interleukin-1 |
| Zdroj: | Arthritis and rheumatism. 41(12) |
| ISSN: | 0004-3591 |
| Popis: | OBJECTIVE To evaluate the sites of expression of interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNFalpha), and inducible nitric oxide synthase (iNOS) in patients with inflammatory and degenerative joint diseases. METHODS Cytokines and iNOS were detected by immunohistochemistry analysis of synovial and cartilage biopsy specimens obtained at knee arthroscopy in patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), osteoarthritis (OA), and traumatic knee arthritis. Cytokine and iNOS expression was quantified using computerized image analysis. RESULTS IL-1beta, TNFalpha, and iNOS were highly expressed by synovial cells (lining layer cells, infiltrating leukocytes, endothelial cells) from patients with inflammatory arthritides and significantly less by synovial cells from patients with OA and traumatic arthritis. In contrast, the latter patients showed high chondrocyte expression of cytokines and iNOS while RA and PsA patients had only minor chondrocyte positivity. In both joint compartments, IL-1beta expression, TNFalpha expression, and iNOS expression were strongly correlated. CONCLUSION The enhanced and coordinated expression of IL-1beta, TNFalpha, and iNOS by chondrocytes strongly supports the hypothesis that chondrocytes are the major site of production of mediators of inflammation in human OA, thus playing a primary role in the pathogenesis of this disease. |
| Databáze: | OpenAIRE |
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