cpsJ gene of Streptococcus iniae is involved in capsular polysaccharide synthesis and virulence
| Autor: | Yue-xuan Chen, Yu-kun Zeng, Kaiyu Wang, Yun Zeng, Yang He, Jun Wang, Defang Chen, Ping Ouyang, Yi Geng |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030106 microbiology Mutant Virulence Microbiology Virulence factor Fish Diseases Gene Knockout Techniques UDPglucose 4-Epimerase 03 medical and health sciences Polysaccharides Animals Streptococcus iniae Molecular Biology Gene Bacterial Capsules Cells Cultured biology Wild type General Medicine biology.organism_classification Ictaluridae 030104 developmental biology Genes Bacterial Mutagenesis Bacteria Catfish |
| Zdroj: | Antonie van Leeuwenhoek. 109:1483-1492 |
| ISSN: | 1572-9699 0003-6072 |
| DOI: | 10.1007/s10482-016-0750-1 |
| Popis: | The capsular polysaccharides are an important virulence factor of Streptococcus iniae, protecting the bacterium from destruction and clearance by the immune system. The cpsJ gene encodes a putative UDP-glucose epimerase involved in the capsule synthesis system. To determine the role of the CpsJ protein in the production of the capsule, a ΔcpsJ mutant was generated and analyzed by comparing its growth performances and virulence with those of the wild type (WT) strain. The ΔcpsJ mutant had longer chains, smaller colonies, and a slower growth rate and decreased optical density than the WT, suggesting that the ΔcpsJ mutant produces less capsular polysaccharide. The ΔcpsJ mutant was more able to adhere to and invaded epithelioma papulosum cyprinid cells (EPCs) when its virulence in vitro was compared with that of the WT, but survived less well in the whole blood of channel catfish. When a channel catfish infection model was used to determine the virulence of the ΔcpsJ mutant in vivo, the mutant caused an increase in survival with the mutant (53.33 %) versus the WT (26.67 %). In summary, mutation of the cpsJ gene influenced both the capsule synthesis and virulence of S. iniae. |
| Databáze: | OpenAIRE |
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