Dynamic maceration of Matricaria chamomilla inflorescences: optimal conditions for flavonoids and antioxidant activity
| Autor: | Simone Vieira Pereira, Rayssa A.S.P. Reis, Danielle Cristina Garbuio, Luís Alexandre Pedro de Freitas |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
Central composite design DPPH medicine.medical_treatment Flavonoid lcsh:RS1-441 01 natural sciences lcsh:Pharmacy and materia medica chemistry.chemical_compound medicine Maceration (wine) Food science General Pharmacology Toxicology and Pharmaceutics Apigenin chemistry.chemical_classification Flavonoids biology 010405 organic chemistry 010401 analytical chemistry Fractional factorial design biology.organism_classification 0104 chemical sciences Matricaria chamomilla chemistry PLANTAS MEDICINAIS Desirability Apigenin-7-glycoside |
| Zdroj: | Revista Brasileira de Farmacognosia, Vol 28, Iss 1, Pp 111-117 (2018) Revista Brasileira de Farmacognosia, Vol 28, Iss 1, Pp 111-117 Revista Brasileira de Farmacognosia, Volume: 28, Issue: 1, Pages: 111-117, Published: JAN 2018 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Revista Brasileira de Farmacognosia v.28 n.1 2018 Revista Brasileira de Farmacognosia Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
| Popis: | The aim of this paper was to study and optimize the dynamic maceration process to obtain Matricaria chamomilla L., Asteraceae, inflorescences extracts with optimum flavonoid content and antioxidant activity using a multivariate approach. Hydroalcoholic extracts were obtained by dynamic maceration in lab scale and the influence of extraction temperature, ratio of plant to solvent, ethanol strength; extraction time and stirring speed on the flavonoid content and antioxidant activity were unveiled using a fractional factorial design. The ethanol strength, ratio of plant to solvent and temperature were the three factors that influenced most the extract properties and were studied by a central composite design. Total flavonoid content and antioxidant activity were affected by the ethanol strength and ranged from 1.49 to 3.95% and 13.3 to 36.2 μg/ml, respectively. The desirability functions resulted in an optimal dynamic maceration condition using 1 h extraction at stirring speed of 900 rpm, ethanol 74.7%, temperature of 69 °C and using 36.8% of plant in solvent (w/v). Under this set of conditions, the extract had total flavonoid content of 4.11 ± 0.07%, in vitro antioxidant activity with IC50 of 18.19 μg/ml and apigenin and apigenin-7-glycoside contents of 2.0 ± 0.1 mg/g and 20.1 ± 0.9 mg/g, respectively. The results showed a low solvent consumption compared to previous works. The model was able to predict extract properties with maximum deviation of 12% and the extraction process developed herein showed to be reliable, efficient and scalable for M. chamomilla inflorescences, enriched with flavonoids, apigenin and apigenin-7-glycoside and high antioxidant activity. Keywords: Flavonoids, DPPH, Apigenin, Apigenin-7-glycoside, Desirability |
| Databáze: | OpenAIRE |
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