Correlation between the Antimicrobial Activity and Metabolic Profiles of Cell Free Supernatants and Membrane Vesicles Produced by Lactobacillus reuteri DSM 17938
| Autor: | Raffaella Muraro, Francesca Sisto, Stefan Roos, Maria Elisa Crestoni, Paola Lanuti, Alessandro Maccelli, Antonella Di Sotto, Helena Bysell, Rossella Grande, Alba Lasalvia, Simone Carradori, Valentina Puca |
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| Rok vydání: | 2020 |
| Předmět: |
Limosilactobacillus reuteri
0301 basic medicine Microbiology (medical) Electrospray ionization Lactobacillus reuteri 030106 microbiology Microbiology biofilm 03 medical and health sciences Minimum inhibitory concentration Virology lcsh:QH301-705.5 antimicrobial activity Minimum bactericidal concentration biology cell free supernatant Chemistry Broth microdilution Metabolism Antimicrobial biology.organism_classification metabolomics extracellular and membrane vesicles lactobacillus reuteri limosilactobacillus reuteri probiotics Microbiology (Microbiology in the medical area to be 30109) 030104 developmental biology lcsh:Biology (General) Biochemistry Bacteria |
| Zdroj: | Microorganisms, Vol 8, Iss 1653, p 1653 (2020) Microorganisms Volume 8 Issue 11 |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms8111653 |
| Popis: | The aim of the work is to assess the antimicrobial activities of Cell Free Supernatants (CFS) and Membrane Vesicles (MVs), produced by Lactobacillus reuteri DSM 17938, versus Gram-positive and Gram-negative bacteria and investigate their metabolic profiles. The Minimum Inhibitory Concentration was determined through the broth microdilution method and cell proliferation assay and the Minimum Bactericidal Concentration was determined by Colony Forming Units counts. The characteristics of the antimicrobial compounds were evaluated by pH adjustments, proteinase treatment, and size fractionation of the CFS. The cytotoxicity of CFS was tested on two human cell lines. A detailed snapshot of the L. reuteri metabolism was attained through an untargeted metabolic profiling by means of high resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) coupled with Electrospray Ionization Source (ESI). The results showed (i) a greater efficacy of CFS and its fractions towards Gram-negative compared to Gram-positive bacteria (ii) an antimicrobial effect related to pH-dependent compounds but not to MVs (iii) a molecular weight < 3 KDa as well as an a non-proteinaceous nature of the antimicrobial compounds and (iv) more than 200 and 500 putative metabolites annotated in MVs and supernatants, covering several classes of metabolites, including amino acids, lipids, fatty and organic acids, polyalcohols, nucleotides, and vitamins. Some putative compounds were proposed not only as characteristic of specific fractions, but also possibly involved in antimicrobial activity. |
| Databáze: | OpenAIRE |
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