Characterization of the Humoral Immune Response to Porcine Reproductive and Respiratory Syndrome (PRRS) Virus Infection
| Autor: | Howard T. Hill, Sabrina L. Swenson, Ken Eernisse, Kyoung-Jin Yoon, Lydia L. Rhinehart, Jeffrey J. Zimmerman, Kenneth B. Platt, Andy Brevik, Merwin L. Frey, Mcginley Michael J |
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| Rok vydání: | 1995 |
| Předmět: |
0301 basic medicine
Swine 040301 veterinary sciences Viral protein Blotting Western Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral medicine.disease_cause Sensitivity and Specificity Virus Immunoenzyme Techniques 0403 veterinary science 03 medical and health sciences Togaviridae Infections Immune system Antigen Antibody Specificity Neutralization Tests Togaviridae medicine Animals Respiratory system Fluorescent Antibody Technique Indirect Antigens Viral Respiratory Tract Infections Swine Diseases General Veterinary Immunoperoxidase Antibody titer Syndrome 04 agricultural and veterinary sciences Virology 030104 developmental biology Antibody Formation biology.protein Female Antibody Genital Diseases Female |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 7:305-312 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/104063879500700302 |
| Popis: | The development of the humoral immune response against porcine reproductive and respiratory syndrome (PRRS) virus was monitored by an indirect fluorescent antibody (IFA) test, immunoperoxidase monolayer assay (IPMA), enzyme-linked immunosorbent assay (ELISA), and serum virus neutralization (SVN) test over a 105-day period in 8 pigs experimentally infected with ATCC strain VR-2402. Specific antibodies against PRRS virus were first detected by the IFA test, IPMA, ELISA, and the SVN test 9-11, 5-9, 9-13, and 9-28 days postinoculation (PI), respectively, and reached their maximum values by 4-5, 5-6, 4-6, and 10-11 weeks PI, respectively, thereafter. After reaching maximum value, all assays showed a decline in antibody levels. Assuming a constant rate of antibody decay, it was estimated by regression analysis that the ELISA, IFA, IPMA, and SVN antibody titers would approach the lower limits of detection by approximately days 137, 158, 324, and 356 PI, respectively. In this study, the immunoperoxidase monolayer assay appeared to offer slightly better performance relative to the IFA test, ELISA, and SVN test in terms of earlier detection and slower rate of decline in antibody titers. Western immunoblot analysis revealed that antibody specific for the 15-kD viral protein was present in all pigs by 7 days PI and persisted throughout the 105-day observation period. Initial detection of antibodies to the 19-, 23-, and 26-kD proteins varied among pigs, ranging from 9 to 35 days PI. Thereafter, the antibody responses to these 3 viral proteins of PRRS virus continued to be detected throughout the 105-day study period. These results clearly indicate that the 15-kD protein is the most immunogenic of the 4 viral proteins identified and may provide the antigenic basis for the development of improved diagnostic tests for the detection of PRRS virus antibodies. |
| Databáze: | OpenAIRE |
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