A new chemical procedure for selective sequential separation of serum lipoproteins
| Autor: | M Schinella, U Lippi, Maria Stella Graziani, F Manzato |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Intermediate-density lipoprotein
Very low-density lipoprotein Chromatography Triglyceride Apolipoprotein B biology Cholesterol Lipoproteins Clinical Biochemistry Hyperlipidemias chemistry.chemical_compound chemistry Biochemistry Mole biology.protein Methods Chemical Precipitation Humans lipids (amino acids peptides and proteins) Chemical composition Triglycerides Chylomicron |
| Zdroj: | La Ricerca in clinica e in laboratorio. 16(4) |
| ISSN: | 0390-5748 |
| Popis: | A new chemical procedure for selective sequential separation of serum lipoproteins is described. Low density lipoproteins (LDL) were precipitated by polyvinylsulfate and the precipitates were redissolved in NaCl 0.45 mol/l. Very low density lipoproteins (VLDL) were precipitated from LDL-free supernatants by PEG-6000 at 100 g/l final concentration, pH 10, and the precipitates were redissolved in NaCl 0.15 mol/l. For the precipitation of high density lipoproteins (HDL), apolipoprotein B-free supernatants were treated by PEG-6000 at 266 g/l final concentration and the precipitates were redissolved in NaCl 0.15 mol/l. The procedure was effective up to a serum triglyceride concentration of 4.0 mmol/l as well as in serum samples without chylomicrons or remnants. The determination of cholesterol and triglyceride contents in separated lipoproteins allows the study of their chemical composition in normo- and moderately hypertriglyceridemic serum samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|