Immunochemical analysis of a photoreceptor protein using anti-IP3 receptor antibody in the unicellular organism, Blepharisma
| Autor: | Akemi Kida, Tomohiko Suzuki, Naoko Moriyama, Kazuo Okuda, Tatsuomi Matsuoka, Hiyoshizo Kotsuki |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cell
Immunoblotting Molecular Sequence Data Biophysics Receptors Cytoplasmic and Nuclear Inositol 1 4 5-Trisphosphate Cross Reactions Unicellular organism medicine Animals Inositol 1 4 5-Trisphosphate Receptors Radiology Nuclear Medicine and imaging Amino Acid Sequence Ciliophora Receptor Fluorescent Antibody Technique Indirect Peptide sequence Perylene Radiation Radiological and Ultrasound Technology biology Photoreceptor protein Pigments Biological Inositol trisphosphate receptor biology.organism_classification Molecular biology Rats medicine.anatomical_structure Biochemistry Blepharisma biology.protein Photoreceptor Cells Invertebrate Calcium Channels Antibody |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology. 54(2-3) |
| ISSN: | 1011-1344 |
| Popis: | The blepharismin–200 kD protein complex of the ciliated protozoan Blepharisma is a novel type of photosensor responsible for the step-up photophobic response of the cell. In immunoblotting assays, the 200 kD protein is weakly cross-reacted with anti-inositol triphosphate receptor antibody (anti-IP 3 R antibody). Indirect immunofluorescence assays show that the pigment granules in which the blepharismin–200 kD protein complex is localized are labelled by anti-IP 3 R antibody. When the anti-IP 3 R antibody or antisense oligonucleotide for IP 3 receptor is introduced into the living cells of Blepharisma , both the photosensitivity of the cells and content of blepharismin–200 kD protein are reduced. The results suggest that the photoreceptor 200 kD protein is possibly an IP 3 receptor-like protein. |
| Databáze: | OpenAIRE |
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