Protective effect of Hedansanqi Tiaozhi Tang against non-alcoholic fatty liver disease in vitro and in vivo through activating Nrf2/HO-1 antioxidant signaling pathway
| Autor: | Mingyang Qiu, Fengqin Xiao, Wu Zhao, Wang Tianning, Songlan Piao, Mingming Yan, Daqing Zhao, Shuai Shao |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Antioxidant NF-E2-Related Factor 2 medicine.medical_treatment Phytochemicals Pharmaceutical Science Hyperlipidemias Salvia miltiorrhiza Pharmacology Diet High-Fat Antioxidants Rats Sprague-Dawley 03 medical and health sciences Mice 0302 clinical medicine Non-alcoholic Fatty Liver Disease 3T3-L1 Cells mental disorders Drug Discovery Hyperlipidemia Nonalcoholic fatty liver disease medicine Lipolysis Animals 030304 developmental biology 0303 health sciences Crataegus Chemistry Fatty liver medicine.disease Complementary and alternative medicine Liver Adipogenesis 030220 oncology & carcinogenesis Heme Oxygenase (Decyclizing) Molecular Medicine Liver function Steatohepatitis Drugs Chinese Herbal |
| Zdroj: | Phytomedicine : international journal of phytotherapy and phytopharmacology. 67 |
| ISSN: | 1618-095X |
| Popis: | Background Hedansanqi Tiaozhi Tang extract (HTT) consists of Notoginseng, Danshen, Hawthorn and Lotus leaf from traditional Chinese medicine, which has significant therapeutic effects on hyperlipidemia in patients with non-alcoholic fatty liver disease (NAFLD). Purpose This study sought to evaluate the pharmacological effects and molecular mechanism of HTT for the treatment of hyperlipidemia in adipocytes and animal model with NAFLD. Methods Quantitative phytochemical analysis of HTT was performed by HPLC. Antioxidant activity and the adipogenesis in 3T3-L1 cells were assessed. In the rat model induced by high-fat diet, lipid-related and antioxidant markers in serum and liver were detected. Moreover, the organ weights, non-alcoholic steatohepatitis (NASH) score and the levels of Nrf2 and HO-1 in liver sections were analyzed by tissue pathological techniques. Results 8 constituents were identified in HTT including saponins, flavonoids, alkaloids and others. HTT treatment enhanced antioxidant activities and promoted lipolysis in 3T3-L1 adipocytes. We also found that HTT inhibited weight gain, reduced the lipid profiles and improved the liver function and pathological characteristics induced by high-fat diet. In addition, HTT activated the Nrf2/HO-1 antioxidant pathway in the liver. Conclusion HTT has protective effect against NAFLD in vitro and in vivo by activating the Nrf2/HO-1 antioxidant pathway. |
| Databáze: | OpenAIRE |
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