Rapid Production and Genetic Stability of Human Mesenchymal Progenitor Cells Derived from Human Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells
| Autor: | Dong Ryul Lee, Soo Kyung Jung, Chang Woo Lee, Jeoung Eun Lee, Sung Han Shim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Nuclear Transfer Techniques
Somatic cell QH301-705.5 Induced Pluripotent Stem Cells Genomic Instability Article Catalysis Cell Line Inorganic Chemistry Gene expression genetic stability Humans Copy-number variation Physical and Theoretical Chemistry Progenitor cell Biology (General) Induced pluripotent stem cell Molecular Biology QD1-999 Cells Cultured Spectroscopy Polymorphism Genetic Chemistry Organic Chemistry Mesenchymal stem cell human mesenchymal progenitor cells Cell Differentiation Mesenchymal Stem Cells General Medicine Computer Science Applications Cell biology differentiation efficiency human SCNT-PSCs Somatic cell nuclear transfer DNA microarray |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 9238, p 9238 (2021) International Journal of Molecular Sciences Volume 22 Issue 17 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Pluripotent stem cell-derived mesenchymal progenitor cells (PSC-MPCs) are primarily derived through two main methods: three-dimensional (3D) embryoid body-platform (EB formation) and the 2D direct differentiation method. We recently established somatic cell nuclear transfer (SCNT)-PSC lines and showed their stemness. In the present study, we produced SCNT-PSC-MPCs using a novel direct differentiation method, and the characteristics, gene expression, and genetic stability of these MPCs were compared with those derived through EB formation. The recovery and purification of SCNT-PSC-Direct-MPCs were significantly accelerated compared to those of the SCNT-PSC-EB-MPCs, but both types of MPCs expressed typical surface markers and exhibited similar proliferation and differentiation potentials. Additionally, the analysis of gene expression patterns using microarrays showed very similar patterns. Moreover, array CGH analysis showed that both SCNT-PSC-Direct-MPCs and SCNT-PSC-EB-MPCs exhibited no significant differences in copy number variation (CNV) or single-nucleotide polymorphism (SNP) frequency. These results indicate that SCNT-PSC-Direct-MPCs exhibited high genetic stability even after rapid differentiation into MPCs, and the rate at which directly derived MPCs reached a sufficient number was higher than that of MPCs derived through the EB method. Therefore, we suggest that the direct method of differentiating MPCs from SCNT-PSCs can improve the efficacy of SCNT-PSCs applied to allogeneic transplantation. |
| Databáze: | OpenAIRE |
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