Effects of Long-term Serial Passaging on the Characteristics and Properties of Cell Lines Derived From Uveal Melanoma Primary Tumors
| Autor: | Arnaud de la Fouchardière, Marjorie-Allison Bergeron, Frédéric Fournier, Arnaud Droit, Mohib W. Morcos, Karine Zaniolo, Solange Landreville, Sylvain L. Guérin, Frédéric Mouriaux, Cindy Weidmann |
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| Přispěvatelé: | CHU Pontchaillou [Rennes], Université de Rennes - Faculté de Médecine (UR Médecine), Université de Rennes (UR), CHU de Québec, Axe cancer, Université Laval [Québec] (ULaval)-CHUQ Research Center, Natural Sciences and Engineering Research Council of Canada (NSERC) [138624-2012], Fondation du CHU de Quebec, Fonds de Recherche du Quebec - Sante (FRQS), Vision Health Research Network (VHRN), Faculte de medecine de l'Universite Laval, Centre de recherche en organogenese experimentale de l'Universite Laval/LOEX, Université de Rennes 1 - Faculté de Médecine (UR1 Médecine), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Uveal Neoplasms
0301 basic medicine short tandem repeat Blotting Western Cell Mice Nude Cell Count [SDV.CAN]Life Sciences [q-bio]/Cancer Biology Polymerase Chain Reaction Metastasis Mice 03 medical and health sciences models MART-1 Antigen Cancer stem cell Cell Line Tumor imatinib mesylate establishment medicine Animals Humans metastasis Microscopy Phase-Contrast RNA Neoplasm ocular melanoma Melanoma cancer stem-cells Gene Expression Profiling human choroidal melanoma Neoplasms Experimental Gene signature [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism medicine.disease Immunohistochemistry gene-expression 3. Good health Gene Expression Regulation Neoplastic Gene expression profiling 030104 developmental biology Imatinib mesylate medicine.anatomical_structure xenografts Cell culture Cancer research Female [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Investigative Ophthalmology & Visual Science Investigative Ophthalmology & Visual Science, 2016, 57 (13), pp.5288--5301. ⟨10.1167/iovs.16-19317⟩ Investigative Ophthalmology & Visual Science, Association for Research in Vision and Ophthalmology, 2016, 57 (13), pp.5288--5301. ⟨10.1167/iovs.16-19317⟩ |
| ISSN: | 0146-0404 1552-5783 |
| DOI: | 10.1167/iovs.16-19317⟩ |
| Popis: | International audience; PURPOSE. Development of liver metastasis remains the most common cause of mortality in uveal melanoma (UM). A few cell lines cultured from primary UM tumors have been used widely to investigate the pathobiology of UM. However, the translation of basic knowledge to the clinic for the treatment of the metastatic disease has remained incremental at best. In this study, we examined whether the properties of UM cell lines at various passages were similar to their corresponding primary tumors. METHODS. Gene expression profiling by microarray was performed on UM primary tumors and derived cell lines cultured at varying passages. Expression of UM protein markers was monitored by immunohistochemical analyses and Western blotting. The in vivo tumorigenic properties of UM cultures were evaluated using athymic nude mice. RESULTS. Cell passaging severely reduced the expression of genes encoding markers typical of UM, including those of the prognostic gene signature. Marked differences between gene expression profiles of primary tumors and cell lines could be linked to the infiltrating immune and stromal cells in situ. In addition, the tumorigenic properties of UM cell lines also increased with cell passaging in culture as evaluated by their subcutaneous injection into athymic mice. CONCLUSIONS. Together, these findings demonstrate that the short-term UM primary cultures exhibit molecular features that resemble the respective surgical material and, thus, represent the best model for in vitro-assessed cancer treatments. |
| Databáze: | OpenAIRE |
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