An experimental solution for the Luria–Delbrück fluctuation problem in measuring hypermutation rates
| Autor: | Jürgen Bachl, Carina Olsson, Charles Steinberg, Mark C. Dessing, R. C. von Borstel |
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| Rok vydání: | 1999 |
| Předmět: |
Genetic Markers
Cell division Green Fluorescent Proteins Mutant Population Somatic hypermutation Biology Transfection medicine.disease_cause Green fluorescent protein Plasmid medicine Humans Computer Simulation education Cell Line Transformed Probability Mutation education.field_of_study Multidisciplinary Models Genetic Molecular biology Stop codon Luminescent Proteins Codon Terminator Commentary |
| Zdroj: | Proceedings of the National Academy of Sciences. 96:6847-6849 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.96.12.6847 |
| Popis: | A cell line harboring all trans-acting elements necessary for hypermutation was transfected with a plasmid harboring the major cis-acting elements plus a green fluorescent protein gene containing a premature chain-termination codon. Transfected cells do not fluoresce unless the stop codon reverts. When a sizable cell population is purged of revertants by sorting, the frequency of mutants increases linearly with time, and there is no Luria–Delbrück fluctuation effect. Moreover, as mutant frequencies seemed to vary less than cell numbers in replicate cultures, it is suggested that hypermutation might not be coupled closely to cell division. |
| Databáze: | OpenAIRE |
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