Heterogenous expression of nestin in human pancreatic tissue precludes its use as an islet precursor marker
| Autor: | Ray V. Rajotte, Cale Street, Lakey, A. M. J. Shapiro, Gregory S. Korbutt, K Seeberger, Lisa M. H. Helms |
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| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Ductal cells Endocrinology Diabetes and Metabolism Cellular differentiation Nerve Tissue Proteins Enteroendocrine cell Vimentin macromolecular substances Biology Polymerase Chain Reaction Nestin Islets of Langerhans Endocrinology Intermediate Filament Proteins Internal medicine medicine Humans RNA Messenger Progenitor cell Pancreas Analysis of Variance Pancreatic Ducts Immunohistochemistry medicine.anatomical_structure Microscopy Fluorescence biology.protein Keratins Biomarkers Adult stem cell |
| Zdroj: | Journal of Endocrinology. 180:213-225 |
| ISSN: | 1479-6805 0022-0795 |
| Popis: | The discovery of a pancreatic adult stem cell would have significant implications for cell-based replacement therapies for type 1 diabetes mellitus. Nestin, a marker for neural precursor cells, has been suggested as a possible marker for islet progenitor cells. We have characterized the expression and localization of nestin in both the intact human pancreas and clinical human pancreatic islet grafts. Nestin was found to be expressed at different levels in the acinar component of human pancreatic biopsies depending on donor, as well as in ductal structures and islets to some degree. In islets, insulin-producing beta-cells rarely co-expressed the protein, and in the ducts a small percentage (1-2%) of cells co-expressed nestin and cytokeratin 19 (CK19) while most expressed only CK19 (90%) or nestin (5-10%) alone. Assessment of nestin expression in neonatal pancreatic sections revealed an increased number of islet-associated positive cells as compared with adult islets. Nestin immunoreactivity was also found in cells of the pancreatic vasculature and mesenchyme as evidenced by co-localization with smooth muscle actin and vimentin. Samples from post-islet isolation clinical islet grafts revealed a pronounced heterogeneity in the proportion of nestin-positive cells ( |
| Databáze: | OpenAIRE |
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