Inter-laboratory comparison of Ki-67 proliferating index detected by visual assessment and automated digital image analysis

Autor: Nives Jonjić, Ilija Jurković, Branka Vrbičić, Loredana Labinac Peteh, Valerija Blažičević, Snježana Tomić, Ivana Mahovne, Sandra Radiković, Jasminka Jakić Razumović, Ivana Mrklić, Nataša Lisica Šikic, Majda Vučić, Božena Šarčević, Dražen Švagelj, Čedna Tomasović Lončarić, Slavko Gašparov
Rok vydání: 2019
Předmět:
0301 basic medicine
Cancer Research
medicine.medical_specialty
BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences
proliferacijski indeks
Proliferative index
Proliferation index
Referral
Croatia
Concordance
Ki 67
Breast Neoplasms
03 medical and health sciences
0302 clinical medicine
Breast cancer
breast cancer
immunohistochemistry
Ki 67
proliferation index
Internal medicine
Image Processing
Computer-Assisted
Medicine
Humans
breast cancer
immunohistochemistry
proliferation index
Ki-67
Cell Proliferation
Automation
Laboratory
Paraffin Embedding
biology
business.industry
rak dojke
BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti
BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences
General Medicine
medicine.disease
Laboratories
Hospital
Immunohistochemistry
imunohistokemija
030104 developmental biology
Cross-Sectional Studies
Ki-67 Antigen
Oncology
030220 oncology & carcinogenesis
biology.protein
Female
Numeric Value
business
BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti
Value (mathematics)
Zdroj: Breast Disease
Volume 1(2019)
ISSN: 1558-1551
0888-6008
Popis: Background: Proliferation rate is a major determinant of the biologic behavior of the tumor and provides information that can be used to guide treatment decisions. Methods: This ring study included 27 pathologists from 14 Institutions, in order to assess inter-observer concordance between pathologists in Croatia. We analyzed Ki-67 proliferative index on ten randomly selected breast cancer samples comparing consistency between visual assessment using light microscopy compared to digital image analyses results from one central laboratory as a referral value. Results: When we analyzed Ki-67 as numeric value high concordance rate was found between Ki-67 score visually assessed in all participating Institutions compared to referral value assessed by digital image analysis (ICC 0.76, 95% CI 0.58-0.91), and Krippendorff's alpha was 0.79 (95% CI 0.58-1.00). Concordance was better in slides with higher Ki-67 values. When we categorized Ki-67 values according to generally accepted 20% cut-off value we noticed the lower concordance rate among participants in our study. Conclusion: Proliferation remains one of the most important parameters for tumor characterization helpful in making clinical decisions, but it should be used with great caution. Standardization of the Ki-67 assessment is essential and proliferating index should be expressed as exact numeric value. For patients with proliferative index near the cut-off value, other factors must be considered in making clinical decisions.
Databáze: OpenAIRE
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