Mammary Tumor Associated RNAs impact tumor cell proliferation, invasion and migration
| Autor: | Kung-Chi Chang, Susan M. Freier, Junyan Song, Frank Rigo, Osama El Demerdash, David L. Spector, C. Frank Bennett, Alexander Krasnitz, Sarah D. Diermeier |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Survival Mammary gland Breast Neoplasms Mammary Neoplasms Animal Mice Transgenic Biology General Biochemistry Genetics and Molecular Biology Article Transcriptome 03 medical and health sciences long non-coding RNAs Mice breast cancer Downregulation and upregulation Cell Movement Cell Line Tumor Spheroids Cellular medicine Animals Humans RNA Neoplasm lcsh:QH301-705.5 Cell Proliferation Gene knockdown Mammary tumor Cell growth Cell migration Molecular biology mammary tumor Long non-coding RNA 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Cancer research 3D organoid culture Female RNA Long Noncoding antisense oligonucleotides Oligoribonucleotides Antisense |
| Zdroj: | Cell Reports, Vol 17, Iss 1, Pp 261-274 (2016) |
| Popis: | SummaryLong non-coding RNAs (lncRNAs) represent the largest and most diverse class of non-coding RNAs, comprising almost 16,000 currently annotated transcripts in human and 10,000 in mouse. Here, we investigated the role of lncRNAs in mammary tumors by performing RNA-seq on tumor sections and organoids derived from MMTV-PyMT and MMTV-Neu-NDL mice. We identified several hundred lncRNAs that were overexpressed compared to normal mammary epithelium. Among these potentially oncogenic lncRNAs we prioritized a subset as Mammary Tumor Associated RNAs (MaTARs) and determined their human counterparts, hMaTARs. To functionally validate the role of MaTARs, we performed antisense knockdown and observed reduced cell proliferation, invasion, and/or organoid branching in a cancer-specific context. Assessing the expression of hMaTARs in human breast tumors revealed that 19 hMaTARs are significantly upregulated and many of these correlate with breast cancer subtype and/or hormone receptor status, indicating potential clinical relevance. |
| Databáze: | OpenAIRE |
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