On-line SPE-CE for the determination of insulin derivatives in biological fluids
| Autor: | N. F. C. Visser, Henk Lingeman, M van Harmelen, Hubertus Irth |
|---|---|
| Přispěvatelé: | BioAnalytical Chemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_treatment
Clinical Biochemistry Relative standard deviation Molecular Sequence Data Pharmaceutical Science Urine Analytical Chemistry Drug Discovery medicine Biological fluids Animals Humans Insulin Technology Pharmaceutical Solid phase extraction Amino Acid Sequence Spectroscopy Chromatography Chemistry Electrophoresis Capillary Repeatability Body Fluids Electrophoresis Cattle SDG 6 - Clean Water and Sanitation Quantitative analysis (chemistry) |
| Zdroj: | Visser, N F C, van Harmelen, M, Lingeman, H & Irth, H 2003, ' SPE-CE for the determination of insulin derivatives in biological fluids ', Journal of Pharmaceutical and Biomedical Analysis, vol. 33, no. 3, pp. 451-62 . https://doi.org/10.1016/S0731-7085(03)00292-9 Journal of Pharmaceutical and Biomedical Analysis, 33(3), 451-62. Elsevier |
| ISSN: | 0731-7085 |
| Popis: | An on-line SPE-CE system is described for the determination of insulin derivatives in urine, serum and plasma. By combining techniques based on different separation mechanisms, in this case reversed-phase SPE and CE, a more selective sample clean-up is obtained. The described on-line SPE-CE procedure is able to desalt and clean biological samples, resulting in more repeatable electrophoretic results as well as a good linearity for urine, serum and plasma samples spiked with insulin derivatives, thus proving the elimination of detrimental effects caused by the sample matrix. The on-line SPE-CE system was linear for urine, serum and plasma samples spiked with insulin derivatives between 5 and 80 mg/l. The repeatability in migration time was below 1% relative standard deviation (R.S.D.). The repeatability of the peak was better ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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