Myofibroblasts enable invasion of endothelial cells into three-dimensional tumor cell clusters: a novel in vitro tumor model
| Autor: | Bruce Pratt, Steve Ledbetter, Jennifer Walter-Yohrling, Beverly A. Teicher |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Cell type Indoles Stromal cell Paclitaxel Angiogenesis Angiogenesis Inhibitors Biology Toxicology Neovascularization Cell–cell interaction Cell Line Tumor Neoplasms medicine Humans Neoplasm Invasiveness Protease Inhibitors Pyrroles Pharmacology (medical) Cells Cultured Pharmacology Endothelial Cells Fibroblasts Antineoplastic Agents Phytogenic Immunohistochemistry Oxindoles Endothelial stem cell Oncology Tumor progression Cell culture Metalloproteases Cancer research Propionates Stromal Cells medicine.symptom |
| Zdroj: | Cancer Chemotherapy and Pharmacology. 52:263-269 |
| ISSN: | 1432-0843 0344-5704 |
| DOI: | 10.1007/s00280-003-0664-2 |
| Popis: | In an effort to study the importance of stromal involvement in angiogenesis, we developed a novel, multicellular model that utilizes three of the primary cell types involved in tumor angiogenesis.Fluorescently labeled human microvascular endothelial cells (HMVECs), 10T1/2 cells and myofibroblasts were incubated in the presence of a three-dimensional tumor cell cluster resuspended in collagen and embedded in Matrigel.HMVECs cultured in the presence of a human SKOV-3 ovarian carcinoma tumor cell cluster, surrounded the tumor cell cluster, while myofibroblasts invaded the cluster, localizing within the tumor cell mass. In contrast, 10T1/2 cells, a pluripotent mouse mesenchymal cell line with pericyte-like properties, did not demonstrate the same invasive phenotype. HMVECs cultured in the presence of myofibroblasts invaded the tumor cell cluster and colocalized with the myofibroblasts as demonstrated by fluorescent microscopy and immunohistochemistry. The angiogenesis inhibitors SU6668 and paclitaxel inhibited stromal invasion, while a broad-spectrum matrix metalloproteinase inhibitor did not.This model emphasizes the critical interaction between endothelial cells and myofibroblasts and provides a more complete in vitro model for studying angiogenesis and tumor progression. |
| Databáze: | OpenAIRE |
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