Shiga Toxin Induces Lipid Compression: A Mechanism for Generating Membrane Curvature
| Autor: | Haifei Gao, Ludger Johannes, Jean-Claude Florent, Eva Y. Chi, Jaroslaw Majewski, Erik B. Watkins |
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| Rok vydání: | 2019 |
| Předmět: |
Models
Molecular Shigella dysenteriae Membrane biology Bioengineering 02 engineering and technology Endocytosis Shiga Toxin Humans General Materials Science Dysentery Bacillary biology Chemistry Mechanical Engineering Phosphatidylethanolamines Trihexosylceramides Cell Membrane Shiga toxin Biological membrane General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Compression (physics) Membrane Membrane curvature Compressibility Biophysics biology.protein 0210 nano-technology |
| Zdroj: | Nano letters. 19(10) |
| ISSN: | 1530-6992 |
| Popis: | Biomembranes are hard to compress laterally, and membrane area compressibility has not been associated with biological processes. Using X-ray surface scattering, we observed that bacterial Shiga toxin compresses lipid packing in a gel phase monolayer upon binding to its cellular receptor, the glycolipid Gb3. This toxin-induced reorganization of lipid packing reached beyond the immediate membrane patch that the protein was bound to, and linkers separating the Gb3 carbohydrate and ceramide moieties modulated the toxin's capacity to compress the membrane. Within a natural membrane, asymmetric compression of the toxin-bound leaflet could provide a mechanism to initiate narrow membrane bending, as observed upon toxin entry into cells. Such lipid compression and long-range membrane reorganization by glycolipid-binding proteins represent novel concepts in membrane biology that have direct implications for the construction of endocytic pits in clathrin-independent endocytosis. |
| Databáze: | OpenAIRE |
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