Oviduct prostacyclin functions as a paracrine factor to augment the development of embryos
| Autor: | Kenneth K. Wu, Farinaz Arbab, Jaou Chen Huang, Nena Aleksic, Ziad Melhem, Jennifer S. Goldsby |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
animal structures Embryonic Development Mice Inbred Strains Prostacyclin Biology Andrology Mice Paracrine signalling Cytochrome P-450 Enzyme System Western blot Pregnancy Internal medicine medicine Animals Cyclooxygenase Inhibitors Blastocyst Fallopian Tubes Nitrobenzenes Sulfonamides Cyclooxygenase 2 Inhibitors medicine.diagnostic_test Rehabilitation Embryogenesis Membrane Proteins Obstetrics and Gynecology Embryo culture Embryo Epoprostenol Intramolecular Oxidoreductases Endocrinology medicine.anatomical_structure Reproductive Medicine Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Cyclooxygenase 1 Eicosanoids Oviduct Female lipids (amino acids peptides and proteins) medicine.drug |
| Zdroj: | Human Reproduction. 19:2907-2912 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/humrep/deh520 |
| Popis: | Recently we discovered that human oviducts produce a significant amount of prostacyclin (prostaglandin I2, PGI2) and that PGI2 enhances the potentials of live birth of mouse embryos. However, the eicosanoid profile of mouse oviducts remains unknown.The metabolites of [14C]arachidonic acid by mouse oviducts were analysed by high-performance liquid chromatography. The expression of cyclooxygenase (COX)-1, COX-2 and PGI2 synthase (PGIS) was analysed by western blot analysis and immunohistochemistry. The PGI2 synthetic capacities and the COX transcripts during the preimplantation period were compared. The effects of COX-2 inhibitor on PGI2 production were ascertained.Mouse oviducts produced, in order of abundance, PGI2, PGD2 and PGE2. Western blot analysis confirmed the expression of COX-1, -2 and PGIS which were expressed by luminal epithelia and smooth muscle cells. Day 2-3 post-coitus (p.c.) oviducts produced PGI2 10-fold higher than day 4 p.c. oviducts (P = 0.0087); day 1 p.c. oviducts expressed COX-2 transcript 5-fold higher than day 3 p.c. oviducts (P = 0.0004). The PGI2 production was markedly reduced by a selective COX-2 inhibitor.Mouse oviducts synthesized maximal PGI2 during day 2-3 p.c., coinciding with the transformation of 2-cell embryos to morulae. The results suggest that oviduct-derived PGI2 may enhance embryo development in a paracrine fashion. |
| Databáze: | OpenAIRE |
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