In vitro characterization of rat bone marrow-derived dendritic cells and their precursors
| Autor: | Y. Wakizaka, Anthony J. Demetris, Conor P. Delaney, T. E. Starzl, J. J. Fung, M. Chen-Woan, Noriko Murase, V. Fournier |
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| Rok vydání: | 1996 |
| Předmět: |
Immunoconjugates
Recombinant Fusion Proteins Immunology Antigen presentation Immunoglobulins Autoimmunity Bone Marrow Cells chemical and pharmacologic phenomena Major histocompatibility complex Article Immune tolerance Abatacept Adjuvants Immunologic Antigen Antigens CD Bone Marrow Immune Tolerance medicine Animals Immunology and Allergy CTLA-4 Antigen CD90 Cells Cultured Antigen Presentation biology Stem Cells Histocompatibility Antigens Class II Antibodies Monoclonal Dendritic Cells Cell Biology Antigens Differentiation Molecular biology Rats Transplantation Phenotype medicine.anatomical_structure Solubility Rats Inbred Lew Antigens Surface biology.protein Bone marrow Lymphocyte Culture Test Mixed CD8 |
| Zdroj: | Journal of Leukocyte Biology. 59:196-207 |
| ISSN: | 1938-3673 0741-5400 |
| Popis: | Although the rat is commonly used for basic immunology and transplantation research, phenotypic and functional characterization of rat dendritic cells (DCs) lags behind similar studies in the human and mouse. Therefore, these features were examined using DCs propagated from cultures of rat bone marrow maintained in a medium supplemented with granulocyte-monocyte colony-stimulating factor. Analysis of cytospin preparations of cultured cells showed that DCs arise from OX7+ myelomonocytic precursors. Typical mature rat DCs were morphologically similar to their mouse and human counterparts and expressed major histocompatibility complex (MHC) class II (common part determinant of Ia), OX62 (integrin molecule), OX7 (CD90), ICAM-1 (CD54), and CTLA4 counterreceptor, but were negative for OX8 (CD8), OX19 (CD5), W3/25 (CD4), and ED2, a rat macrophage marker. Functional analysis of OX62+ sorted DCs showed that they could effectively present the soluble antigen ovalbumin to naive T cells in vitro. A combination of anti-MHC class II monoclonal antibody and CTLA4-immunoglobulin inhibited allostimulatory ability more effectively than either reagent alone. Implications for studying the role of DCs in immune responses in the rat are discussed. |
| Databáze: | OpenAIRE |
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