COMPARATIVE STUDY OF THE 17-KETOGENIC (NORYMBERSKI), GLENN-NELSON AND REDDY METHODS FOR THE DETERMINATION OF C21URINARY STEROIDS
| Autor: | Orville J. Golub, Charles Sobel, Richard J. Henry |
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| Rok vydání: | 1958 |
| Předmět: |
Pregnanetriol
medicine.medical_specialty Endocrinology Diabetes and Metabolism ADRENAL CORTICOSTEROIDS Urinary system Biochemistry (medical) Clinical Biochemistry Residual urine Urine Biochemistry Body Fluids chemistry.chemical_compound Endocrinology chemistry Adrenal Cortex Hormones Internal medicine Adrenogenital syndrome medicine Humans |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 18:522-530 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-18-5-522 |
| Popis: | A series of urine samples was analyzed for 17-OH-corticosteroids by the 17-ketogenic steroid method of Norymberski, the Glenn-Nelson method and the Reddy technique. The results indicate relatively good correlation between the Norymberski and Glenn-Nelson values and the clinical findings, but serious discrepancies were obtained with the Reddy method, especially in urines containing only small amounts of corticosteroids. Utilizing 3 wavelengths for reading with the latter procedure, although frequently altering the values, did not necessarily improve their agreement with those obtained by the other 2 methods nor with the final diagnosis. Data are presented which indicate that the Glenn-Nelson technique leaves a significant percentage of the 17-OH-corticosteroids in the residual urine following hydrolysis and extraction. The results point up the advantage of the Norymberski procedure in the diagnosis of the adrenogenital syndrome, a condition in which pregnanetriol is excreted in abnormal amounts and measure... |
| Databáze: | OpenAIRE |
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