Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn’s Disease

Autor: Nora Sipeki, Stefan Rödiger, Mandy Sowa, Dirk Roggenbuck, Uwe Roesler, Mária Papp, Martin W. Laass, Jayaseelan Murugaiyan, Dimitrios P. Bogdanos, Nadja Röber, Karsten Conrad, Claudia Deutschmann, Peter Schierack
Rok vydání: 2019
Předmět:
Adult
Male
Crohn’s disease
0301 basic medicine
Immunoglobulin A
Adolescent
Neutrophils
Blotting
Western
Immunoglobulins
Enzyme-Linked Immunosorbent Assay
600 Technik
Medizin
angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
Inflammatory bowel disease
CHI3L1
Immunoglobulin G
Antibodies
Antineutrophil Cytoplasmic
03 medical and health sciences
0302 clinical medicine
Crohn Disease
Antigen
Germany
medicine
Humans
Chitinase-3-Like Protein 1
Child
Autoantibodies
biology
business.industry
chitinase-3 like protein 1
Gastroenterology
Autoantibody
Original Articles
General Medicine
medicine.disease
030104 developmental biology
Case-Control Studies
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Immunology
biology.protein
Female
030211 gastroenterology & hepatology
Antibody
business
Biomarkers
autoantibody
Zdroj: Journal of Crohn's & Colitis
ISSN: 1876-4479
1873-9946
DOI: 10.1093/ecco-jcc/jjz012
Popis: Background and AimsThere is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated.MethodsAutoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs].ResultsThe 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD.ConclusionCHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.
Databáze: OpenAIRE
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