Novel natural spider silk embedded electrospun nanofiber mats for wound healing
| Autor: | Neşe Kurt Özkaya, Kerim Emre Öksüz, Zeynep Deniz Şahin İnan, Ali Özer |
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| Přispěvatelé: | Tıp Fakültesi, Mühendislik Fakültesi |
| Rok vydání: | 2021 |
| Předmět: |
Vinyl alcohol
Materials science Biocompatibility 02 engineering and technology 010402 general chemistry 01 natural sciences Extracellular matrix chemistry.chemical_compound Electrospun nanofibers Materials Chemistry General Materials Science Spider silk Electrospinning integumentary system Adhesion Silk fibers 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Mechanics of Materials Surgical wounds Nanofiber Wound dressing 0210 nano-technology Wound healing Biomedical engineering |
| Popis: | In recent years, electrospun nanofiber mats based on biopolymers have been extensively investigated for tissue and biomedical engineering, mainly because of remarkable morphological similarity with the natural extracellular matrix. The current study focuses on the preparation of novel natural spider silk (SS) embedded Poly(vinyl alcohol)(PVA)/Sodium alginate (NaAlg) wound dressings with desirable properties for a wound dressing application. The nanofibers' surface morphology and structure were observed by a field emission scanning electron microscope (FE-SEM). In-vivo evaluation of PVA/NaAlg based electrospun nanofiber mats as a wound dressing material and their comparison to commercially available wound dressings was carried out by in-vivo tests on rabbit models. Some morphometric parameters such as counting cells, blood vessels, endothelial cells, determination of the area of the wound closure, wound healing performance, speed of wound healing and collagen thickness were investigated using OM and FE-SEM post-processing. Furthermore, in-vitro biocompatibility and cellular behavior such as adhesion and proliferation of mouse fibroblast cells (L929) were studied by XTT assay on developed nanofiber mats. The results of this experimental study indicated that the natural spider silk embedded electrospun nanofiber mat (PVA/NaAlg/SS) accelerated the rate of wound healing compared to other groups by improving the collagen formation rate, proliferative cell activity as well as decreasing the inflammatory cell amount. Furthermore, the results of the in-vivo and in-vitro experiments suggest that novel PVA/NaAlg/SS nanofiber mats might be a fascinating bioactive wound dressing for clinical applications. |
| Databáze: | OpenAIRE |
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