Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction

Autor:	Montserrat Shelbourne, Xiong Chen, Afaf H. El-Sagheer, Tom Brown
Rok vydání:	2011
Předmět:	Azides Base pair Stereochemistry Oligonucleotides Alkyne 010402 general chemistry Photochemistry 01 natural sciences Catalysis Ring strain chemistry.chemical_compound Materials Chemistry Fluorescent Dyes chemistry.chemical_classification DNA ligase 010405 organic chemistry Metals and Alloys DNA General Chemistry Cycloaddition 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry Cyclization Alkynes Ceramics and Composites Click Chemistry Azide Ligation Copper
Zdroj:	Chemical Communications; Vol 47 Chemical Communications
ISSN:	1364-548X 1359-7345
DOI:	10.1039/c1cc10743g
Popis:	Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c24b2adb00794a411cf8fefa3139189f https://doi.org/10.1039/c1cc10743g Zobrazit plný text záznamu