Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene
| Autor: | Nikolaj Blom, Thomas Sicheritz-Pontén, Søren Rysgaard, Simon Rasmussen, Jeff S. Bowman, Jody W. Deming |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Biodiversity
ice cover genetic analysis phylogeny Arctic RNA Ribosomal 16S genetics Ice Cover species richness Bacteria (microorganisms) cladistics Phylogeny biodiversity geography.geographical_feature_category Ecology Arctic Regions article High-Throughput Nucleotide Sequencing methodology bacterium sea ice niche Original Article microbial community Spartobacteria archaeon RNA 16S sea water Biology Cyanobacteria Microbiology high throughput sequencing cyanobacterium Sea ice Seawater Ecology Evolution Behavior and Systematics geography Bacteria Phylum isolation and purification microbiology multiyear sea ice Arctic ice pack Archaea Microbial population biology gene expression Pyrosequencing 454 pyrosequencing Species richness sea ice bacteria |
| Zdroj: | Bowman, J S, Rasmussen, S, Blom, N, Deming, J W, Rysgaard, S & Sicheritz-Ponten, T 2012, ' Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene ', I S M E Journal, vol. 6, no. 1, pp. 11-20 . https://doi.org/10.1038/ismej.2011.76 |
| ISSN: | 1751-7370 |
| DOI: | 10.1038/ismej.2011.76 |
| Popis: | Dramatic decreases in the extent of Arctic multiyear ice (MYI) suggest this environment may disappear as early as 2100, replaced by ecologically different first-year ice. To better understand the implications of this loss on microbial biodiversity, we undertook a detailed census of the microbial community in MYI at two sites near the geographic North Pole using parallel tag sequencing of the 16S rRNA gene. Although the composition of the MYI microbial community has been characterized by previous studies, microbial community structure has not been. Although richness was lower in MYI than in underlying surface water, we found diversity to be comparable using the Simpson and Shannon's indices (for Simpson t=0.65, P=0.56; for Shannon t=0.25, P=0.84 for a Student's t-test of mean values). Cyanobacteria, comprising 6.8% of reads obtained from MYI, were observed for the first time in Arctic sea ice. In addition, several low-abundance clades not previously reported in sea ice were present, including the phylum TM7 and the classes Spartobacteria and Opitutae. Members of Coraliomargarita, a recently described genus of the class Opitutae, were present in sufficient numbers to suggest niche occupation within MYI. © 2012 International Society for Microbial Ecology All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|