Duplication and Loss of Function of Genes Encoding RNA Polymerase III Subunit C4 Causes Hybrid Incompatibility in Rice
| Autor: | Hiroyuki Kanamori, Yuko Shigematsu, Yuta Miyazaki, Yoshiyuki Yamagata, Takashi Matsumoto, Giao N. Nguyen, Satoru Kuhara, Jianzhong Wu, Atsushi Yoshimura, Kazuyuki Doi, Kosuke Tashiro, Hideshi Yasui, Miyako Watanabe |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Time Factors Genetic Linkage QH426-470 01 natural sciences hybrid sterility chemistry.chemical_compound Gene Expression Regulation Plant Chromosome Segregation Gene Duplication RNA polymerase Gene duplication Cloning Molecular Genetics (clinical) Plant Proteins Genetics education.field_of_study Chromosome Mapping food and beverages Pollen nonfunctionalization Heterozygote Plant Infertility Genotype Sterility Population Germination Investigations Biology Genes Plant 03 medical and health sciences Genetic linkage reproductive barrier Allele education Molecular Biology Gene Crosses Genetic neutral gene Oryza sativa RNA Polymerase III Epistasis Genetic Oryza Protein Subunits Fertility 030104 developmental biology chemistry Hybridization Genetic genetic drift 010606 plant biology & botany |
| Zdroj: | G3: Genes|Genomes|Genetics SC30201810100004 NARO成果DBa 書誌情報のみ G3: Genes, Genomes, Genetics, Vol 7, Iss 8, Pp 2565-2575 (2017) |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.117.043943 |
| Popis: | Reproductive barriers are commonly observed in both animals and plants, in which they maintain species integrity and contribute to speciation. This report shows that a combination of loss-of-function alleles at two duplicated loci, DUPLICATED GAMETOPHYTIC STERILITY 1 (DGS1) on chromosome 4 and DGS2 on chromosome 7, causes pollen sterility in hybrid progeny derived from an interspecific cross between cultivated rice, Oryza sativa, and an Asian annual wild rice, O. nivara. Male gametes carrying the DGS1 allele from O. nivara (DGS1-nivaras) and the DGS2 allele from O. sativa (DGS2-T65s) were sterile, but female gametes carrying the same genotype were fertile. We isolated the causal gene, which encodes a protein homologous to DNA-dependent RNA polymerase (RNAP) III subunit C4 (RPC4). RPC4 facilitates the transcription of 5S rRNAs and tRNAs. The loss-of-function alleles at DGS1-nivaras and DGS2-T65s were caused by weak or nonexpression of RPC4 and an absence of RPC4, respectively. Phylogenetic analysis demonstrated that gene duplication of RPC4 at DGS1 and DGS2 was a recent event that occurred after divergence of the ancestral population of Oryza from other Poaceae or during diversification of AA-genome species. |
| Databáze: | OpenAIRE |
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