Repression of P53-Dependent Sequence-Specific Transactivation by MEF2C
| Autor: | Teruyo Tsukada, Shinichi Aizawa, Mariko Nagayoshi, Yoji Ikawa, Hiromi Sato, Mitsuo Kato |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Transcriptional Activation
DNA Complementary Transcription Genetic Immunoblotting Molecular Sequence Data Biophysics Clone (cell biology) Gene Expression MADS Domain Proteins Spleen Biology Transfection Biochemistry Cell Line Mice Transactivation Consensus Sequence medicine Animals Humans MEF2C Molecular Biology Gene Psychological repression Transcription factor Gene Library Base Sequence MEF2 Transcription Factors cDNA library Cell Biology Genes p53 Molecular biology Mice Mutant Strains Recombinant Proteins DNA-Binding Proteins medicine.anatomical_structure Myogenic Regulatory Factors Tumor Suppressor Protein p53 Oligonucleotide Probes Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 214:468-474 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1995.2310 |
| Popis: | Lambda ZAP cDNA library constructed from spleen of a p53-deficient mouse was screened by South-Western technique using Fragment A, a DNA sequence that p53 specificaly binds to, as a probe. One (clone 2) of six clones isolated was identical to MEF2c, a MADS-family transcription factor. Transcripts of the mef2c gene was also detected in spleen where the expression has not been reported so far. Isolated clones except clone 2 had a growth-suppression activity on p53-deficient osteosarcoma cell line (Saos II). Clone 2 repressed the transactivation from Fragment A by p53, suggesting that MEF2c may act as a negative regulator of p53-responsive element. |
| Databáze: | OpenAIRE |
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