The mycotoxins deoxynivalenol and nivalenol show in vivo synergism on jejunum enterocytes apoptosis
| Autor: | Maria Vilariño, Juliette Cognie, Philippe Pinton, Patrick Callu, Sophal Cheat, Anne Marie Cossalter, Isabelle P. Oswald, Isabelle Raymond-Letron, Martine Kolf-Clauw |
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| Přispěvatelé: | ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Faculty of Animal Science and Veterinary Medicine, Royal University of Agriculture (RUA), Biosynthèse & Toxicité des Mycotoxines (ToxAlim-BioToMyc), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Plateforme Ezop (Ezop), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Pôle Valorisation Animale, ARVALIS - Institut du végétal [Paris], Institut National Polytechnique, STROMALab, Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Etablissement Français du Sang-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR DON & Co project, Université Fédérale Toulouse Midi-Pyrénées, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement Français du Sang-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine pig Swine deoxynivalenol Pharmacology Toxicology human health Jejunum chemistry.chemical_compound Intestinal mucosa 2. Zero hunger santé animale apoptosis General Medicine santé humaine 3. Good health medicine.anatomical_structure [SDV.TOX]Life Sciences [q-bio]/Toxicology Female fusariotoxins mycotoxine Enterocyte interaction Biology maladie intestinale entérocyte 03 medical and health sciences In vivo medicine Animals porcin jejunum Mycotoxin Cell Proliferation intussusception apoptose animal health Cell growth nivalénol barrière intestinale Enterocytes 030104 developmental biology intestinal mucosa loops chemistry Apoptosis Immunology Trichothecenes Food Science Contaminated food |
| Zdroj: | Food and Chemical Toxicology Food and Chemical Toxicology, Elsevier, 2016, 87, pp.45-54. ⟨10.1016/j.fct.2015.11.019⟩ |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2015.11.019⟩ |
| Popis: | Remerciements : Plate-forme CIRE Chirurgie et Imagerie pour la Recherche et l’Enseignement, INRA, UMR PRC 37380 Nouzilly, Centre Val de Loire; The mycotoxins deoxynivalenol (DON) and nivalenol (NIV), worldwide cereal contaminants, raise concerns for human and animal gut health, following exposure through contaminated food and feed. The aim of this work was to analyze the effects of DON and NIV, alone or associated, on the intestinal pig mucosa. Jejunal loops were used for testing DON and NIV individually and in combination (1:1) after a single exposure, for 24 hours. For repeated exposure, piglets received a natural contaminated feed, with DON or with DON+NIV for 28 days. Histological investigations, proliferation and apoptosis assessments were conducted. Both experiments were concordant for the total-cell proliferation decreased at the villus tips after DON or DON+NIV at 10 μM acutely, or repeatedly, by 30-35% and 20-25%, respectively. In loops model, apoptotic enterocytes at villus tips increased dose-dependently after DON, NIV alone or DON+NIV in combination. The combination in loops at 10 μM showed higher effects on proliferation and apoptosis than DON alone, and synergism was shown for villus apoptotic enterocyte. These results are to be considered for NIV consumer risk assessment. Our results demonstrate the in vivo disruption of the intestinal balance proliferation/apoptosis explaining, at least partly, the disruption of intestinal barrier by these mycotoxins. |
| Databáze: | OpenAIRE |
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