Synthesis and transfection activity of novel galactosylated polycationic lipid
| Autor: | Marina A. Zenkova, Rapoport Da, Roman N. Serikov, G. A. Serebrennikova, N. G. Morozova, D.A. Medvedeva, Mikhail A. Maslov, Valentin V. Vlassov |
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| Rok vydání: | 2011 |
| Předmět: |
Cell Survival
Transgene Clinical Biochemistry Pharmaceutical Science Spermine Microscopy Atomic Force Transfection Biochemistry Cell Line chemistry.chemical_compound Cricetinae Drug Discovery Polyamines Animals Humans Molecular Biology Molecular Structure Oligonucleotide Organic Chemistry Genetic transfer Gene Transfer Techniques Galactose Biological activity Flow Cytometry Lipids Polyelectrolytes chemistry Liposomes Nucleic acid Molecular Medicine lipids (amino acids peptides and proteins) DNA |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 21:2937-2940 |
| ISSN: | 0960-894X |
| Popis: | In this study, we synthesized a new galactosylated cationic lipid and investigated its biological activity. The structure of lipid combines both spermine residue for DNA compaction and galactose moiety for the improvement of aggregation behavior of lipoplexes. Lipid was low toxic for different mammalian cells, and was able both to compact plasmid DNA and to mediate cellular accumulation of various nucleic acids (ODN, pDNA and siRNA) exhibiting biological activity (transgene expression, gene silencing). |
| Databáze: | OpenAIRE |
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