CKS proteins protect mitochondrial genome integrity by interacting with mitochondrial single-stranded DNA-binding protein
| Autor: | Mark Crawford, Marko Radulovic, Jasminka Godovac-Zimmermann, Eleanor Crane, Veronica P. C. C. Yu |
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| Rok vydání: | 2009 |
| Předmět: |
Mitochondrial DNA
Immunoblotting DNA Single-Stranded Cell Cycle Proteins Plasma protein binding Mitochondrion Biochemistry DNA-binding protein DNA Mitochondrial Mass Spectrometry Analytical Chemistry Cell Line Mitochondrial Proteins Cyclin-dependent kinase Cell Line Tumor CDC2 Protein Kinase CDC2-CDC28 Kinases Animals Humans Electrophoresis Gel Two-Dimensional Phosphorylation Cell Cycle Protein Molecular Biology Cyclin-dependent kinase 1 biology Research Cyclin-Dependent Kinases Recombinant Proteins Cell biology Mitochondria DNA-Binding Proteins Microscopy Fluorescence Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Genome Mitochondrial Mutation biology.protein RNA Interference biological phenomena cell phenomena and immunity Carrier Proteins Protein Kinases Mitochondrial DNA replication Protein Binding |
| Zdroj: | Molecularcellular proteomics : MCP. 9(1) |
| ISSN: | 1535-9484 |
| Popis: | Cyclin-dependent kinase subunit (CKS) proteins interact with cyclin-dependent kinases (CDKs) with high affinity. Mammalian CKS1 and CKS2 bind CDK1 and CDK2 and partake in the control of cell cycle progression. We identified CKS-interacting proteins by affinity purification followed by mass spectrometry in the human lymphocytic cell line Ramos. Apart from known interactors, such as CDKs, we identified a novel CDK-dependent interaction between CKS proteins and the mitochondrial single-stranded DNA-binding protein (mtSSB). mtSSB bound both CKS1 and CKS2 and underwent CDK-dependent phosphorylation. mtSSB is known to participate in replication of mitochondrial DNA. We demonstrated that mitochondrial morphology and DNA integrity were compromised in cells depleted of both CKS proteins or that had inhibited CDK activity. These features are consistent with the hypothesis of CKS-dependent regulation of mtSSB function and support a direct role of cell cycle proteins in controlling mitochondrial DNA replication. |
| Databáze: | OpenAIRE |
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