Expression and the role of 3'-phosphoadenosine 5'-phosphosulfate transporters in human colorectal carcinoma
| Autor: | Tatsuro Irimura, Yuzuru Ikehara, Tomomi Ichimiya, Shin Kamiyama, Hayao Nakanishi, Takeshi Suda, Shoko Nishihara, Shoji Nakamori, Hisashi Narimatsu, Izumi Fujimoto, Masahiko Watanabe, Fumiaki Nakayama, Mitsuru Nakamura, Tomofumi Takase |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Anion Transport Proteins
Phosphoadenosine Phosphosulfate Gene Expression Fibroblast growth factor Biochemistry Trimethoprim symbols.namesake chemistry.chemical_compound Sulfation Polysaccharides RNA interference Tumor Cells Cultured medicine Humans Gene silencing Neoplasm Invasiveness RNA Messenger Cell Proliferation Sulfamonomethoxine Chemistry Membrane Transport Proteins Cancer Biological Transport Epithelial Cells Transporter Fibroblasts Golgi apparatus medicine.disease Immunohistochemistry Cell biology Drug Combinations 3'-Phosphoadenosine-5'-phosphosulfate Sulfate Transporters symbols RNA Interference Colorectal Neoplasms Signal Transduction |
| Zdroj: | Glycobiology. 21:235-246 |
| ISSN: | 1460-2423 0959-6658 |
| DOI: | 10.1093/glycob/cwq154 |
| Popis: | Sulfation represents an essential modification for various molecules and regulates many biological processes. The sulfation of glycans requires a specific transporter for 3'-phosphoadenosine 5'-phosphosulfate (PAPS) on the Golgi apparatus. This study investigated the expression of PAPS transporter genes in colorectal carcinomas and the significance of Golgi-specific sulfation in the proliferation of colorectal carcinoma cells. The relative amount of PAPST1 transcripts was found to be higher than those of PAPST2 in colorectal cancerous tissues. Immunohistochemically, the enhanced expression of PAPST1 was observed in fibroblasts in the vicinity of invasive cancer cells, whereas the expression of PAPST2 was decreased in the epithelial cells. RNA interference of either of the two PAPS transporter genes reduced the extent of sulfation of cellular proteins and cellular proliferation of DLD-1 human colorectal carcinoma cells. Silencing the PAPS transporter genes reduced fibroblast growth factor signaling in DLD-1 cells. These findings indicate that PAPS transporters play a role in the proliferation of colorectal carcinoma cells themselves and take part in a desmoplastic reaction to support cancer growth by controlling their sulfation status. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|