New incompatibilities uncovered using the Promega DNA IQ™ chemistry
| Autor: | Della Wilkinson, Brian Yamashita, Chantal J. Frégeau, Florence Célestin, Nancy Laurin, Meagan Clark |
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| Rok vydání: | 2015 |
| Předmět: |
Dna integrity
Chromatography Chemistry DNA Molecular biology DNA extraction DNA Fingerprinting Latent fingerprint Pathology and Forensic Medicine Magnetic powder chemistry.chemical_compound DNA profiling Humans Centrifugation Indicators and Reagents Ink Fingerprint powder Dermatoglyphics Powders Law Blood Chemical Analysis Microsatellite Repeats |
| Zdroj: | Forensic science international. 257 |
| ISSN: | 1872-6283 |
| Popis: | Over the years, the Promega DNA IQ™ System was proven an effective technology for the production of clean DNA from a wide variety of casework specimens. The capture of DNA using the DNA IQ™ paramagnetic beads, however, was shown to be affected by a few specific chemicals that could be present on exhibits submitted to the laboratory. In this study, various blood and latent fingerprint enhancement reagents/methods, marker pens and adhesive tapes, applied at the crime scene or in the forensic laboratory on casework exhibits or used to collect biological material, were tested for their compatibility with the DNA IQ™ technology. Although no impact on DNA recovery was observed for most reagents, the MAGNA™ Jet Black fingerprint powder and three 3M Scotch(®) adhesive tapes were shown to severely or completely inhibit DNA binding onto the DNA IQ™ beads. The effect of MAGNA™ Jet Black on DNA recovery could be counteracted by separating the magnetic powder from the lysates by centrifugation or filtration, prior to DNA extraction. High quality STR profiles were obtained from samples subjected to MAGNA™ Jet Black suggesting it does not impact DNA integrity. |
| Databáze: | OpenAIRE |
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