Activation of T cells by dendritic cells exposed to a reference sensitizer Towards a promising model to assess the allergenic potential of chemicals
| Autor: | Fabrice Battais, Isabelle Sponne, Isabelle Langonné, F. Gagnaire, Christophe Paris, Julianne Mathiot, Hakima Abdessadeq, Samuel Muller, Cécile Huppert |
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| Přispěvatelé: | Interactions Gènes-Risques environnementaux et Effets sur la Santé (INGRES), Université de Lorraine (UL), Département Toxicologie et biométrologie (TB), Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) (INRS ( Vandoeuvre lès Nancy)), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Allergy medicine.medical_treatment T cells Platinum Compounds Dermatology Lymphocyte Activation 03 medical and health sciences Mice Chlorides Surface marker medicine Immunology and Allergy Animals dendritic cells Alternative methods [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health Mice Inbred BALB C Chemistry fungi Limiting Allergens medicine.disease Flow Cytometry 030104 developmental biology Cytokine in vitro assay chemical allergens Cancer research Cytokines Cell response Cytokine secretion Female Biomarkers |
| Zdroj: | Contact Dermatitis Contact Dermatitis, Wiley, 2018, 79 (2), pp.67-75. ⟨10.1111/cod.12991⟩ Contact Dermatitis, 2018, 79 (2), pp.67-75. ⟨10.1111/cod.12991⟩ |
| ISSN: | 0105-1873 1600-0536 |
| Popis: | International audience; Background - Low molecular weight chemicals constitute one of the major causes of occupational allergies. European legislation on chemicals recommends limiting the use of in vivo models for assessing the sensitizing potential of chemicals, and encourages the development of integrated alternative methods. An in vitro mouse model of bone marrow-derived dendritic cells (BMDCs) that showed good accuracy (75%) and sensitivity (69%) has previously been developed to assess the sensitizing potential of chemicals. Objective - To assess the ability of BMDCs to activate T cells (TCs) in vitro. Methods - BMDCs pre-exposed to the reference sensitizer ammonium hexachloroplatinate (AHCP) were co-cultured with different subpopulations of TCs. TC activation was assessed by surface marker expression, proliferation, and cytokine release. Results - The results showed significant activation of TCs co-cultured with dendritic cells pre-exposed to AHCP as evaluated by CD124 expression, proliferation, and cytokine secretion. Moreover, the response of TCs appeared to be Th2-oriented. Naive TCs were shown to be involved in this response, and the removal of regulatory TCs did not improve the cell response. Conclusions - The BMDCs used in this previously developed model appear to have the ability to activate TCs, confirming that the BMDC model represents a reliable assay for assessing the sensitizing potential of chemicals. |
| Databáze: | OpenAIRE |
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