Dietary Cows’ Milk Protein A1 Beta-Casein Increases the Incidence of T1D in NOD Mice
| Autor: | Christophe Lefevre, Karen M. Dwyer, Sonja Kukuljan, Jennifer L. McRae, Joanne S.J. Chia, Ashwantha Kumar Enjapoori |
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| Rok vydání: | 2018 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine medicine.medical_specialty type 1 diabetes Regulatory T cell cows’ milk lcsh:TX341-641 030209 endocrinology & metabolism Nod Biology T-Lymphocytes Regulatory Article 03 medical and health sciences 0302 clinical medicine Mice Inbred NOD Risk Factors Internal medicine Diabetes mellitus Casein medicine Animals Glucose homeostasis Genetic Predisposition to Disease Cells Cultured NOD mice Type 1 diabetes Nutrition and Dietetics epigenetics Caseins beta-casein medicine.disease Animal Feed Coculture Techniques Gastrointestinal Microbiome Diabetes Mellitus Type 1 030104 developmental biology medicine.anatomical_structure Endocrinology Dietary Supplements Animal Nutritional Physiological Phenomena Female Gene-Environment Interaction lcsh:Nutrition. Foods and food supply Insulitis Biomarkers Food Science |
| Zdroj: | Nutrients, Vol 10, Iss 9, p 1291 (2018) Nutrients Volume 10 Issue 9 |
| ISSN: | 2072-6643 |
| Popis: | The contribution of cows&rsquo milk containing beta-casein protein A1 variant to the development of type 1 diabetes (T1D) has been controversial for decades. Despite epidemiological data demonstrating a relationship between A1 beta-casein consumption and T1D incidence, direct evidence is limited. We demonstrate that early life exposure to A1 beta-casein through the diet can modify progression to diabetes in non-obese diabetic (NOD) mice, with the effect apparent in later generations. Adult NOD mice from the F0 generation and all subsequent generations (F1 to F4) were fed either A1 or A2 beta-casein supplemented diets. Diabetes incidence in F0&ndash F2 generations was similar in both cohorts of mice. However, diabetes incidence doubled in the F3 generation NOD mice fed an A1 beta-casein supplemented diet. In F4 NOD mice, subclinical insulitis and altered glucose handling was evident as early as 10 weeks of age in A1 fed mice only. A significant decrease in the proportion of non-conventional regulatory T cell subset defined as CD4+CD25&minus FoxP3+ was evident in the F4 generation of A1 fed mice. This feeding intervention study demonstrates that dietary A1 beta-casein may affect glucose homeostasis and T1D progression, although this effect takes generations to manifest. |
| Databáze: | OpenAIRE |
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