Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1
| Autor: | Tetsuji Yamada, Kazuo Kato, Mutsushi Matsuyama, Takashi Kuramoto, Makoto Kuroda, Kyoko Higo-Moriguchi |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Pathology Thymoma Genotype Lymphocyte education Congenic Biology Animals Congenic Internal medicine medicine Animals Genetic Predisposition to Disease Inbreeding Rats Inbred BUF Hematology Strain (chemistry) Rat strain Heterozygote advantage Thymus Neoplasms General Medicine medicine.disease Phenotype Molecular biology Rats Rats Inbred ACI Disease Models Animal medicine.anatomical_structure Oncology Female |
| Zdroj: | Journal of Cancer Research and Clinical Oncology. 134:789-792 |
| ISSN: | 1432-1335 0171-5216 |
| Popis: | To confirm the presence of the susceptible gene for the thymoma development in the region that was assumed by the previous linkage study by Oyabu et al. (J Natl Cancer Inst 91:279–282, 1999), we tried to establish a congenic strain of rats. Backcrossings between the BUF/Mna strain as a donor strain and the ACI/NMna strain as an inbred partner were repeated for 12 generations, examining whether rats had the thymoma development region, and then homozygous rats were yielded by mating among the heterozygotes. To detect the phenotypic expression, heterozygous ACI.BUF/Mna-Tsr1/+ (ACI-Tsr1/+) rats were generated by crossing female ACI.BUF/Mna-Tsr1/Tsr1 (ACI-Tsr1/Tsr1) rats with male ACI/NMna rats and were maintained for 24 months. These ACI-Tsr1/+ rats produced thymoma in 71%, showing a dominant trait. The thymomas were of the lymphocyte predominant type, as those developed in rats of the original BUF/Mna strain. Thus, a new rat congenic strain, ACI-Tsr1/Tsr1, was established, revealing that thymoma develops in the dominant trait in ACI-Tsr1/+ rats. |
| Databáze: | OpenAIRE |
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