Investigation of the stability of polyelectrolyte multilayer coatings in open‐tubular capillary electrochromatography using laser scanning confocal microscopy

Autor: Kapnissi‐Christodoulou, Constantina P., Lowry, M., Agbaria, R. A., Geng, L., Warner, I. M.
Přispěvatelé: Kapnissi‐Christodoulou, Constantina P. [0000-0003-3755-1052]
Rok vydání: 2005
Předmět:
Osmosis
Laser scanning confocal microscopy
Materials science
synthesis
Surface Properties
surfactant
polymer
Clinical Biochemistry
Analytical chemistry
chirality
confocal laser microscopy
engineering.material
Electrochemistry
poly(diallyldimethylammonium chloride)
Biochemistry
Analytical Chemistry
Surface-Active Agents
Drug Stability
Coating
Pulmonary surfactant
chemical procedures
1
1' binaphthyl 2
2' dihydrogenphosphate
controlled study
Polyelectrolyte multilayer
reproducibility
electroosmosis
polyelectrolyte
separation technique
Chromatography
Micellar Electrokinetic Capillary
Capillary electrochromatography
Microscopy
Confocal
Bilayer
article
Cationic polymerization
Proteins
capillary electrochromatography
1
1' binaphthyl derivative
Polyelectrolyte
unclassified drug
Anionic addition polymerization
Open-tubular capillary electrochromatography
Chemical engineering
chemical analysis
polymerization
chemical structure
engineering
poly(sodium n undecanoyl leucylvalinate)
Zdroj: Electrophoresis
ISSN: 1522-2683
0173-0835
DOI: 10.1002/elps.200410256
Popis: A simple polyelectrolyte multilayer (PEM) coating procedure was used for the development of stable modified capillaries. PEM coatings were constructed in fused-silica capillaries using alternating rinses of cationic and anionic polyelectrolytes. The multilayer coatings investigated in this study consisted of two and twenty layer pairs, or bilayers. A bilayer is one layer of a cationic polymer and one layer of an anionic polymer. Poly(diallyldimethylammonium chloride) was used as the cationic polymer, and the polymeric surfactant poly(sodium N-undecanoyl-L-leucylvalinate) was used as the anionic polymer. Previous studies for both chiral and achiral separations have shown that PEM-coated capillaries have excellent reproducibilities, remarkable endurance, and strong stabilities against extreme pH values when used in open-tubular capillary electrochromatography (OT-CEC). In this study, the stability of the coatings was further investigated after exposure to 0.1 M and 1.0 M NaOH. Structural changes of these coatings were monitored using laser scanning confocal microscopy (LSCM) after flushing the capillaries with NaOH. This technique allowed observation of the degradation of the coatings. Observations are discussed in terms of separations using OT-CEC. Electropherograms obtained from the chiral separation of 1,1'-binaphthyl-2,2'-dihydrogenphosphate in OT-CEC showed a decrease in selectivity and an increase in electroosmotic mobility after long exposure to NaOH. The ability to recover the capillaries by exposure to NaOH was also demonstrated. Measurements of electroosmotic mobility and selectivity showed that 2-bilayer and 20-bilayer PEM coatings could be completely removed from the capillary surface after approximately 3.5 and 9.5 h, respectively, of continuous exposure to 1 M NaOH. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. 26 4-5 783 789 Cited By :22
Databáze: OpenAIRE
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