Sources and implications of basal nitric oxide in spontaneous contractions of guinea pig taenia caeci
| Autor: | M. Teresa Mitjavila, Catalina Caballero-Alomar, P. Puig-Parellada, Diego López, Carmen Santos |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
Nitroprusside medicine.medical_specialty Colon Physiology Guinea Pigs Tetrodotoxin Nitric Oxide Nitric oxide Guinea pig Contractility chemistry.chemical_compound Basal (phylogenetics) Physiology (medical) Internal medicine medicine Spontaneous contraction Animals Nitric Oxide Donors heterocyclic compounds Enzyme Inhibitors Hepatology biology musculoskeletal neural and ocular physiology Electron Spin Resonance Spectroscopy Gastroenterology Muscle Smooth Longitudinal muscle biology.organism_classification Molecular biology NG-Nitroarginine Methyl Ester Endocrinology nervous system chemistry Taenia Nitric Oxide Synthase Muscle Contraction |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 285:G747-G753 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.00273.2002 |
| Popis: | We examined in vitro the source and role of basal nitric oxide (NO) in proximal segments of guinea pig taenia caeci in nonadrenergic, noncholinergic (NANC) conditions. Using electron paramagnetic resonance (EPR), we measured the effect of the NO synthase inhibitor NG-nitro-l-arginine methyl ester (l-NAME, 10–4 M), the neuronal blocker tetrodotoxin (TTX, 10–6 M), or both on spontaneous contractions and on the production of basal NO. Both l-NAME and TTX, when tested alone, increased the amplitude and frequency of contractions. NO production was abolished by l-NAME and was inhibited by 38% by TTX. When tested together, l-NAME in the presence of TTX or TTX in the presence of l-NAME had no further effect on the amplitude or frequency of spontaneous contractions, and the NO production was inhibited. These findings suggest that basal NO consists of TTX-sensitive and TTX-resistant components. The TTX-sensitive NO has an inhibitory effect on spontaneous contractions; the role of TTX-resistant NO is unknown. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|