Switching from HPLC/UV to MEIA for whole blood sirolimus quantitation: comparison of methods
| Autor: | B Poppi, Valentina Ruggieri, Daria Brovia, Diego Pinetti, Daniela Gallesi, Carmela Nives Castellana, Stefania Pisa, Luigi Alberto Pini, Alfio Bertolini |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Ultraviolet Rays Clinical Biochemistry sirolimus rapamycin high-performance liquid chromatography microparticle enzyme immunoassay therapeutic drug monitoring Microparticle Enzyme Immunoassay High-performance liquid chromatography Immunoenzyme Techniques Transplantation Immunology medicine Humans Immunology and Allergy Statistical analysis Chromatography High Pressure Liquid Whole blood Sirolimus Chromatography medicine.diagnostic_test Chemistry Biochemistry (medical) Public Health Environmental and Occupational Health Reproducibility of Results Original Articles Hematology Medical Laboratory Technology Therapeutic drug monitoring Renal transplant Transplant patient Drug Monitoring Blood Chemical Analysis Immunosuppressive Agents medicine.drug |
| Zdroj: | Journal of Clinical Laboratory Analysis. 20:239-244 |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/jcla.20148 |
| Popis: | Sirolimus is a immunosuppressive agent for renal transplant recipients. Monitoring of whole blood sirolimus concentration is necessary in order to improve clinical outcomes. An increasing number of clinical laboratories (4–14% during 2005) are using microparticle enzyme immunoassay (MEIA) for sirolimus quantitation but previous reports indicated a high variability, with a mean difference of 17% for MEIA method vs. high‐performance liquid chromatography/ultraviolet (HPLC/UV). This study was aimed at comparing the reliability of MEIA with the HPLC/UV method. Blood samples from transplant patients were processed using both HPLC/UV and MEIA assays. Comparison and Bland‐Altman plots, as well as regression analysis and paired t‐test were used to compare results of the assays. Concentrations were stratified into three groups and used to investigate whether any observed difference between methods could be influenced by sirolimus concentration. Regression analysis yielded a coefficient of correlation R of 0.9756, the line of best fit being y=0.9832x+0.1976. The statistical analysis showed no difference between the two sets of experimental data. The average percentage difference between the two methods was found to be –0.2±19.2%. On the basis of our present results, the tested MEIA assay is able to quantify sirolimus concentration with a clinically acceptable imprecision, similar to that of HPLC/UV method. J. Clin. Lab. Anal. 20:239–244, 2006. © 2006 Wiley‐Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|