Bacteroides fragilis Enterotoxin Upregulates Intercellular Adhesion Molecule-1 in Endothelial Cells via an Aldose Reductase-, MAPK-, and NF-κB–Dependent Pathway, Leading to Monocyte Adhesion to Endothelial Cells
| Autor: | Su Hyuk Ko, Jung Mogg Kim, Young Jeon Kim, Do Young Yoo, Hyun Cheol Roh |
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| Rok vydání: | 2011 |
| Předmět: |
Endothelium
Immunology Intercellular Adhesion Molecule-1 IκB kinase Biology Monocytes Cell Line Bacteroides fragilis Enterotoxins chemistry.chemical_compound Downregulation and upregulation Aldehyde Reductase Cell Adhesion medicine Animals Humans Immunology and Allergy Mitogen-Activated Protein Kinase Kinases Aldose reductase NF-kappa B Endothelial Cells NF-κB Adhesion Bacteroides Infections Rats Up-Regulation Cell biology medicine.anatomical_structure chemistry Signal transduction Signal Transduction |
| Zdroj: | The Journal of Immunology. 187:1931-1941 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Enterotoxigenic Bacteroides fragilis (ETBF) produces a ∼20-kDa heat-labile enterotoxin (BFT) that plays an essential role in mucosal inflammation. Although a variety of inflammatory cells is found at ETBF-infected sites, little is known about leukocyte adhesion in response to BFT stimulation. We investigated whether BFT affected the expression of ICAM-1 and monocytic adhesion to endothelial cells (ECs). Stimulation of HUVECs and rat aortic ECs with BFT resulted in the induction of ICAM-1 expression. Upregulation of ICAM-1 was dependent on the activation of IκB kinase (IKK) and NF-κB signaling. In contrast, suppression of AP-1 did not affect ICAM-1 expression in BFT-stimulated cells. Suppression of NF-κB activity in HUVECs significantly reduced monocytic adhesion, indicating that ICAM-1 expression is indispensable for BFT-induced adhesion of monocytes to the endothelium. Inhibition of JNK resulted in a significant attenuation of BFT-induced ICAM-1 expression in ECs. Moreover, inhibition of aldose reductase significantly reduced JNK-dependent IKK/NF-κB activation, ICAM-1 expression, and adhesion of monocytes to HUVECs. These results suggest that a signaling pathway involving aldose reductase, JNK, IKK, and NF-κB is required for ICAM-1 induction in ECs exposed to BFT, and may be involved in the leukocyte–adhesion cascade following infection with ETBF. |
| Databáze: | OpenAIRE |
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