Molecular characterization and expression profiling of ryanodine receptor gene in the pink stem borer, Sesamia inferens (Walker)
Autor: | Cong-Fen Gao, Dan-Dan Zhao, Li-Qi Zhou, Si-Qi Zhao (赵思琪), Shun-Fan Wu, Jing-Mei Huang |
---|---|
Rok vydání: | 2018 |
Předmět: |
Male
0106 biological sciences 0301 basic medicine Mannosyltransferase DNA Complementary Health Toxicology and Mutagenesis 01 natural sciences Insecticide Resistance Open Reading Frames 03 medical and health sciences Complementary DNA Animals Amino Acid Sequence RNA Messenger Gene Phylogeny Genetics Sequence Homology Amino Acid biology Ryanodine receptor Gene Expression Profiling Ryanodine Receptor Calcium Release Channel General Medicine biology.organism_classification Lepidoptera Gene expression profiling 010602 entomology Open reading frame Transmembrane domain 030104 developmental biology Female Agronomy and Crop Science Sesamia inferens |
Zdroj: | Pesticide Biochemistry and Physiology. 146:1-6 |
ISSN: | 0048-3575 |
DOI: | 10.1016/j.pestbp.2018.01.004 |
Popis: | The susceptibilities of three field populations of pink stem borer (PSB), Sesamia inferens (walker) to diamide insecticides, chlorantraniliprole and flubendiamide, were evaluated in this study. The results showed that these PSB field populations were still sensitive to the two diamide insecticides after many years of exposure. To further understand PSB and diamide insecticide, the full-length ryanodine receptor (RyR) cDNA (named as SiRyR), the molecular target of diamide insecticides was cloned from PSB and characterized. The SiRyR gene contains an open reading frame of 15,420 nucleotides, encoding 5140 amino acid residues, which shares 77% to 98% sequence identity with RyR homologous of other insects. All hallmarks of RyR proteins are conserved in the SiRyR protein, including the conserved C-terminal domain with the consensus calcium-biding EF-hands (calcium-binding motif), the six transmembrane domains, as well as mannosyltransferase, IP3R and RyR (pfam02815) (MIR) domains. Real-time qPCR analysis revealed that the highest mRNA expression levels of SiRyR were observed in pupa and adults, especially in males. SiRyR was expressed at the highest level in thorax, and the lowest level in wing. The full genetic characterization of SiRyR could provide useful information for future functional expression studies and for discovery of new insecticides with selective insecticidal activity. |
Databáze: | OpenAIRE |
Externí odkaz: |