Identification and expression analysis of a fibrinogen alpha chain-like gene in Atlantic salmon (Salmo salar)
Autor: | Xiaohao Li, Kun-peng Fan, Peng-fei Liu, Yu-qing Xia, Ying Liu, Jian-xin Cheng, Yanyun Liu |
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Rok vydání: | 2022 |
Předmět: |
Innate immunity
Innate immune system medicine.diagnostic_test animal diseases Salmo salar Fibrinogen SH1-691 Aquatic Science Biology biology.organism_classification Molecular biology Aeromonas salmonicida Open reading frame Aquaculture. Fisheries. Angling medicine Animal Science and Zoology Gene expression Salmo Gene Fibrinogen alpha chain Fluorescence in situ hybridization medicine.drug |
Zdroj: | Aquaculture Reports, Vol 22, Iss, Pp 100919-(2022) |
ISSN: | 2352-5134 |
Popis: | Fibrinogen is a protein involved in platelet aggregation that is essential for the coagulation of blood, and it plays a role in the fish immune system. In this study, a fibrinogen alpha chain-like gene of the Atlantic salmon (Salmo salar) (designated Ss-FGA) was cloned, and its expression pattern was analyzed after fish were infected by the bacterium Aeromonas salmonicida. The Ss-FGA gene contains a 135 base pair (bp) 5′-untranslated region (UTR), an open reading frame (ORF) of 1512 bp that encodes a 503 amino acid polypeptide, and a 624 bp 3′-UTR. The expression level of Ss-FGA was higher in the liver than in other tissues, indicating that the liver is the major organ for protein synthesis of Ss-FGA. Additionally, expression levels of Ss-FGA differed between treatment and control samples. The results of FISH (Fluorescence in situ hybridization) showed that the expression of Ss-FGA gene in the liver tissue of Atlantic salmon after infected by A. salmonicida. In conclusion, this study revealed that Ss-FGA might play important roles in the innate immune responses to bacterial invasion in Atlantic salmon. |
Databáze: | OpenAIRE |
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